凋亡调节蛋白XIAP与Omi/HtrA2在弥漫大B细胞淋巴瘤中的表达及其与患者预后的关系

Expression of apoptosis-regulating proteins XIAP and Omi/HtrA2 in diffuse large B-cell lymphoma and relationship with prognosis

目的探讨XIAP与Omi/HtrA2在弥漫大B细胞淋巴瘤(DLBCL)组织中的表达及与其预后的相关性。方法收集59例DLBCL患者和30例淋巴结反应增生(RHL)患者的石蜡病理切片,应用免疫组化法对XIAP和Omi/HtrA2进行染色,分析两种蛋白的表达与DLBCL的临床参数和预后的关系。结果XIAP与Omi/HtrA2在DLBCL中均呈高表达,在RHL中呈低表达甚至未见表达。XIAP在DLBCL的平均阳性率为61.02%,在RHL平均阳性率为36.67%(P<0.05)。Omi/HtrA2在DLBCL中平均阳性率为52.54%,RHL平均阳性率为30.00%(P<0.05)。二者的阳性表达与DLBCL的临床分期、病理类型、乳酸脱氢酶(LDH)水平、淋巴瘤国际预后指数(IPI)评分相关(P<0.05),与性别、年龄、症状分组、结外受累无关(P>0.05)。XIAP和Omi/HtrA2表达呈负相关(P<0.05)。Kaplan-meier生存分析显示,XIAP的表达与DLBCL患者的预后相关(P<0.05)。Omi/HtrA2与DLBCL预后不相关(P>0.05)。结论XIAP与Omi/HtrA2在DLBCL组织中均呈高表达,二者呈负相关,上调Omi/HtrA2抑制XIAP表达可为DLBCL靶向治疗提供依据,XIAP是DLBCL预后相关性因素,有望成为判断DLBCL预后的指标。

Objective To investigate expression and prognosis of XIAP and Omi/HtrA2 in diffuse large B cell lymphoma(DLBCL)and the relationship with prognosis.Methods Paraffin sections were collected from 59 patients with DLBCL and 30 patients with lymph node hyperplasia(RHL).The XIAP and Omi/HtrA2 proteins in both groups were stained by immunoh is to chemical method,the relationship of their expressions with clinical parameters and prognosis of DLBCL were analyzed.Results XIAP and Omi/HtrA2 were highly expressed in DLBCL,but lower expressed or even not expressed in RHL.The average positive rate of XIAP in DLBCL was 61.02%,and that of RHL was 36.67%(P<0.05).The average positive rate of Omi/HtrA2 in DLBCL was 52.54%,and that of RHL was 30.00%(P<0.05).The positive expressions of XIAP and Omi/HtrA2 were associated with clinical stage,pathological type,LDH level,and IPI score of DLBCL(P<0.05),but were not associated with gender,age,extranodal involvement,and symptom grouping(P>0.05).The expressions of XIAP and Omi/HtrA2 were negatively correlated(P<0.05).Kaplan-meier survival analysis showed that the expression of XIAP was correlated with the prognosis of patients with DLBCL(P<0.05),but the expression of Omi/HtrA2 was not correlated with the prognosis of DLBCL patients(P>0.05).Conclusion XIAP and Omi/HtrA2 are both highly expressed in DLBCL,and there are negatively correlated.The upregulation of Omi/HtrA2 and the suppression of XIAP expression can provide a basis for DLBCL targeted therapy.XIAP,a prognostic factor related to DLBCL,will expect to be an indicator of DLBCL prognosis.