上海市宝山区中年人群红细胞参数参考区间的建立

Establishment of reference interval for erythrocyte parameters among middle-aged people in Baoshan area in Shanghai

目的建立该实验室中年(40~55岁)人群红细胞参数的生物参考区间。方法根据美国临床和实验室标准化协会C28-A2指南,选取来该院健康体检的20例中年人群作为研究对象,其中男、女各10例,对其检测数据中的红细胞参数[红细胞分布宽度(RDW)、RBC、平均红细胞体积(MCV)、红细胞分布宽度指数、Green和King指数、Eng和Fraser指数(EFI)、Metzer指数、Srivastava指数(SI)、Shine和Lal指数(SLI)、低血红蛋白密度、小红细胞贫血因子(MAF)]进行正态性分布检验,将离群值剔除,建立并验证参考区间。结果男、女性红细胞比容、MCV和RBC,以及男性红细胞平均血红蛋白含量、Hb、EFI和SI和女性SLI、MAF呈正态分布,其他参数呈偏态分布,经t检验发现,男女间仅RDW差异无统计学意义(t=1.96,P=0.40),其他参数指标差异均有统计学意义(P<0.05)。Z值计算结果显示,RDW、MCV、SLI参数的参考区间可以合并,其他参数需要根据性别给出不同的参考区间。结论新建的生物参考区间更符合中年人群,能更好地指导临床诊断和治疗。

Objective To establish the biological reference intervals for the red blood cell parameters among middle-aged people(40-55 year-old)in the laboratory.Methods According to the CLSI C28-A2 guide,a total of 20 middle-aged persons of physical examination in the hospital were selected,and the red cell parameters,including red blood cell distribution width(RDW),red blood cell(RBC),mean corpuscular volume(MCV),red blood cell distribution width index,Green and King index,Eng and Fraser index(EFI),Metzer index,Srivastava index(SI),Shine and Lal index(SLI),low hemoglobin density,small cell anemia factor(MAF)were tested and the outliers were eliminated,and the reference interval was established and verified.Results Hematocrit,MCV and RBC in both male and female,MCH,Hb,EFI and SI in male and SLI and MAF in female conformed to a Gaussian distribution,and the other parameters were not.The t tests showed that the difference of RDW between male and female was not statistically significant(t=1.96,P=0.4),while the differences of other parameters were statistically significant(P<0.05).The Z value shows that the reference intervals of RDW,MCV and SLI parameters can be combined,and other parameters need to be given different reference intervals according to gender.Conclusion The new biological reference interval is more suitable for the middle-aged people and can guide the clinical diagnosis and treatment effectively.