定量分析幽门螺杆菌根除前后部分肠道菌群的改变

Quantitative Analysis of Major Shifts in Gut Microbiota Before and After Helicobacter pylori Eradication

背景:全球约半数人群存在幽门螺杆菌(Hp)感染,根除Hp是治疗其感染相关疾病的重要方法。目的:分析Hp根除前后部分肠道菌群的改变,探讨铋剂四联疗法对肠道微生态的影响。方法:纳入2016年1月—2017年1月在深圳市第三人民医院发现Hp感染阳性、接受铋剂四联疗法根除治疗并成功根除Hp的个体,采集其Hp根除前和根除治疗结束后3 d内的粪便标本,提取基因组DNA,采用菌属特异性引物行real-time PCR定量分析。结果:与根除前相比,Hp根除后粪便肠杆菌属、肠球菌属数量明显增加,双歧杆菌属、拟杆菌属、梭菌属和总菌数量明显减少,差异均有统计学意义(P<0.05),乳酸杆菌属数量无明显变化(P>0.05)。结论:采用铋剂四联疗法根除Hp可影响肠道菌群结构,导致肠道微生态失衡。

Background:Helicobacter pylori(Hp)infection occurs in about half of the world’s population,and Hp eradication therapy is an important method for treating Hp infection-related diseases.Aims:To explore the major shifts in gut microbiota before and after Hp eradication by using bismuth-containing quadruple therapy.Methods:Fifty Hp-positive patients in whom the Hp was successfully eradicated by bismuth-containing quadruple therapy from January 2016 to January 2017 at the Third People’s Hospital of Shenzhen were enrolled.Using genus-specific primers and genomic DNA extracted from the fecal samples collected before and within 3 days after eradication therapy,a quantitative analysis of the major shifts in gut microbiota was accomplished by real-time PCR.Results:Compared with those before Hp eradication,fecal Enterobacterium and Enterococcus were significantly increased and Bifidobacterium,Bacteroides,Clostridium and total bacteria were significantly decreased after Hp eradication(P<0.05).No significant change was observed in Lactobacillus(P>0.05).Conclusions:Eradication of Hp infection by bismuth-containing quadruple therapy may cause changes in gut microbiota composition and resulting in dysbiosis.