雄性大鼠盆神经损伤神经源性膀胱动物模型的建立

Establishment of a neurogenic bladder animal model through pelvic nerve injury in male rats

目的建立盆神经损伤后神经源性膀胱的大鼠模型,研究引起神经源性膀胱的相关因素。方法将16只雄性SD大鼠采用随机数字表法分为神经损伤组和假手术组,每组8只,神经损伤组损伤双侧盆神经,假手术组仅显露神经但不做处理。4周后行膀胱功能检测,并留取膀胱组织行Masson染色及VAChT阳性神经纤维免疫荧光染色。结果术后4周,神经损伤组大鼠膀胱质量(123.6±16.8)mg较假手术组(87.2±11.2)mg显著增加(P<0.05)。神经损伤组大鼠膀胱最大容量(3.45±0.45)mL较假手术组(1.46±0.11)mL显著增加(P<0.05)。神经损伤组大鼠膀胱逼尿肌收缩力明显下降,最大排尿压峰值为(12.1±6.5)mmHg,明显低于假手术组(42.9±10.5)mmHg(P<0.05)。Masson染色示神经损伤组大鼠膀胱壁组织中平滑肌含量减少,胶原纤维增生明显,其胶原占比(19.75±5.46)%显著高于假手术组(9.95±3.25)%,(P<0.05)。免疫荧光结果显示神经损伤组膀胱壁内VAChT阳性神经纤维占比(7.46±1.68)%较假手术组(12.67±3.24)%显著减少(P<0.05)。结论损伤大鼠双侧盆神经可成功建立神经源性膀胱动物模型,表现为膀胱逼尿肌纤维化,VAChT阳性神经纤维减少及排尿功能受损。

Objective To establish a rat model of neurogenic bladder after pelvic nerve injury and study the relatedfactors causing neurogenic bladder.Methods Sixteen male Sprague Dawley rats were randomly divided into the nerve injury group and the sham operation group(n=8per group).Rats in the nerve injury group underwent bilateralpelvic nerve injury.Rats in the sham group un derwent sham operation.Four weeks postoperatively,the bladder function was detected,and the bladder tissues were harvested for Masson staining andVAChT immunofluorescence staining.Results At 4 weeks postoperatively,bladder weight was significantly in creased in the nerve injury group(123.6±16.8)mg compared with the sham group(87.2±11.2)mg(P<0.05).The maximum blad der volume in the nerve injury group(3.45±0.45)mL was significantly higher than that in the sham operation group(1.46±0.11)mL(P<0.05).The voiding function of rats was significantly decreased in the nerve injury group.The peak urinary pressure of the injured group was(12.1±6.5)mmHg,which was significantly less than that in the sham group(42.9±10.5)mmHg(P<0.05).Mas son staining of the bladder tissue showed a decrease of smooth muscle cells and an intensification of collagen in nerve injury group,and the proportion of collagen(19.75±5.46)%was obviously higher than that in sham group(9.95±3.25)%(P<0.05).Immunoflu orescence staining showed that the proportion of VAChT positive nerve fibers of the bladder wall in the nerve injury group(7.46±1.68)%was significantly less than that in the sham group(12.67±3.24)%(P<0.05).Conclusion A neurogenic bladder animal model can be successfully established through bilateral pelvic nerve injury in male rats,characterized by bladder detrusor fibrosis,VAChT positive nerve fiber reductionand impaired voiding function.