BAY 11-7082对人鼻咽癌细胞增殖和凋亡的影响

Effects of BAY 11-7082 on proliferation and apoptosis of human nasopharyngeal carcinoma cells

目的探讨BAY 11-7082对人鼻咽癌细胞增殖和凋亡的影响。方法人鼻咽癌细胞SUME-a采用2.5 mol/L和5μmol/L的Bay 11-7082培养2 h(实验1组与实验2组),处理时间为48 h,将在细胞当中只加入相同量0.1%DMSO溶液设置为空白对照组。CCK8法检测三组的细胞增殖,采用末端脱氧核苷酸转移酶介导的d-UTP缺口末端标记测定(TUNEL)法检测细胞凋亡率,通过流式细胞仪检测细胞周期,RT-PCR与蛋白免疫印迹(Western blot)法检测YBX-1表达。结果与空白对照组比较,实验1组与实验2组的细胞增殖活性明显降低,而细胞凋亡率明显增加,而实验2组的细胞增殖活性降低更明显,细胞凋亡率增加更明显(P<0.05);随着处理时间的延长,细胞凋亡明显,而实验2组的更明显(P<0.05);与空白对照组比较,实验1组与实验2组的p65、YBX-1表达及p65mRNA、YBX-1mRNA降低,实验2组的p65、YBX-1表达及p65mRNA、YBX-1mRNA降低更明显(P<0.05)。结论BAY11-7082可能抑制人鼻咽癌细胞SUME-a增殖,且具有剂量依赖性,其诱导细胞凋亡,部分机制可能是通过调节p65表达、细胞周期分布与YBX-1蛋白水平而实现。

Objective To investigate the effect of BAY 11-7082 on proliferation and apoptosis of human nasopharyngeal carcinoma cells.Methods Human nasopharyngeal carcinoma cell line SUME-a was cultured in nutrient fluid with Bay 11-7082 of 2.5 mol/L and 5μmol/L for 2 h(experimental group 1 and 2),and the treatment time was 48 hours,and blank control group was set up at the same time,only 0.1%DMSO solution was added to the nutrient fluid.The cell proliferation was detected by CCK8 method,and the apoptosis rate was detected by terminal deoxynucleotidyl transferase-mediated d-UTP Nick end labeling method(TUNEL).Cell cycle was detected by flow cytometry,and the expression of YBX-1 was detected by RT-PCR and Western blot.Results Compared with the blank control group,the cell proliferation activity of experiment group 1 and experimental group 2 decreased,however,the apoptosis rate increased,what’s more the decrease of cell proliferation activity and increase of cell apoptosis rate of experimental 2 group was more obvious(P<0.05).With the prolongation of the treatment time,the apoptosis of the cells was obvious,and that of the experimental group 2 was more obvious(P<0.05).Compared with the blank control group,the expression of p65,YBX-1 and p65 mRNAs in experimental group 1 and experimental group 2 decreased,while the expression of YBX-1 and p65 mRNAs in experimental group 2 decreased even more brightly than that in experimental group 1(P<0.05).Conclusions BAY11-7082 may inhibit the proliferation of human nasopharyngeal carcinoma cell line SUME-a in a dose-dependent manner.It could induce apoptosis in human nasopharyngeal carcinoma cell line SUME-a in a dose-dependent manner,partly by regulating the expression of p65,cell cycle distribution and the level of YBX-1 protein.