蝴蝶兰‘红天鹅’组织培养及快繁技术

Tissue Culture and Rapid Propagation of Phalaenopsis ‘Red Swan’

[目的]探索‘红天鹅’花梗的不定芽诱导、丛生芽继代增殖和生根诱导技术。[方法]以蝴蝶兰新品种‘红天鹅’的花梗作为外植体,开展组织培养技术研究。[结果]用0.1%的HgCl2消毒外植体20 min,污染率可降低至27.2%,萌芽率达91.0%;不定芽继代增殖培养的最适培养基为MS+6-BA 6.0 mg/L+NAA 0.1 mg/L+AD 5.0 mg/L+香蕉50 g/L+土豆50g/L+蔗糖30g/L+琼脂7.5 g/L,增殖系数达3.80;最适合的生根诱导培养基为1/2MS+NAA0.4 mg/L+香蕉50 g/L+土豆50g/L+C 1.0 g/L+蔗糖15 g/L+卡拉胶7.0 g/L,生根时间为15 d,生根率达100%。[结论]发现了适宜‘红天鹅’的从消毒到增殖,再到生根中各试剂和激素的合理用量,为蝴蝶兰快繁技术发展提供了参考。

[Objective] To explore the techniques of adventitious bud induction,cluster bud subculture and rooting induction of ’red swan’pedicel,so as to lay a foundation for the popularization and application of new Phalaenopsis varieties. [Methods] The pedicel of a new Phalaenopsis variety’red swan’was used as explant to study the tissue culture technology. [Results] The results showed that when the explants were sterilized with 0.1% HgCl2 for 20 minutes,the contamination rate was reduced to 27.2%,and the germination rate was 91.0%.The optimum medium for adventitious bud subculture was MS+6-BA 6.0 mg/L+NAA 0.1 mg/L+AD 5.0 mg/L+banana 50 g/L +potato 50 g/L +sucrose 30 g/L +agar 7.5 g/L,and the proliferation coefficient was 3.80. The most suitable medium for rooting induction was 1/2 MS+NAA 0.4 mg/L+banana 50 g/L+potato 50 g/L+C 1.0 g/L +sucrose 15 g/L +carrageenan 7.0 g/L,rooting time was 15 days,rooting rate was 100%. [Conclusion] This study had put forward reasonable dosage of each reagent and hormone among disinfection,proliferation and rooting,and provided reference to development of Phalaenopsis’red swan’rapid propagation.