水稻C24-甾醇甲基转移酶基因OsSMT2的生物信息学分析

Bioinformatics Analysis of C24-Sterol Methyltransferase Gene OsSMT2 in Rice

【目的】为水稻C-24甾醇甲基转移酶基因OsSMT2的进一步研究提供理论参考。【方法】以水稻OsSMT2基因为研究对象,通过生物信息学分析方法,对OsSMT2蛋白质的理化性质、结构特点、共表达基因的富集、启动子的顺式作用元件及表达模式等进行分析。【结果】水稻OsSMT2基因CDS全长1092 bp,编码1个含363个氨基酸的蛋白质,该蛋白质具有甲基转移酶结构域和甾醇甲基转移酶C末端结构域;OsSMT2蛋白质不含信号肽,存在1个跨膜结构域;OsSMT2基因的共表达基因主要富集到DNA的复制过程和次生代谢物的生物合成等途径中,可能参与植物的抗寒和抗旱过程;OsSMT2基因在萌发种子中的表达量最高,在叶片的表达量最低。【结论】水稻OsSMT2基因主要在萌发的种子、胚根、胚芽中高表达,参与水稻DNA复制和细胞分裂的主要过程,可作为水稻抗寒和抗旱育种的候选基因。

【Objective】This paper aimed to provide a reference for the further study of rice C24-sterol methyltransferase 2(OsSMT2)gene.【Method】The physicochemical properties,structural characteristics,enrichment analysis of co-expressed genes,cis-acting elements of pro-moters and expression patterns of OsSMT2 were analyzed.【Result】The CDs of OsSMT2 gene was 1092 bp,encoding a protein of 363 amino acid with a methyltransferase domain and a C-terminal sterol methyltransferase domain.The OsSMT2 gene contained no signal peptide,and had a transmembrane domain.GO and KEGG Pathway Enrichment Analysis showed that the co-expressed genes of OsSMT2 were mainly en-riched in the process of DNA replication and biosynthesis of secondary metabolites.Analysis of the promoter cis-elements revealed that the OsSMT2 maight be involved in the cold and drought resistance in plants.Expression pattern analysis showed that OsSMT2 had the highest ex-pression in the germinated seeds and the lowest expression in the leaves.【Conclusion】OsSMT2 is mainly expressed in germinated seeds,radicle and germ,and participates mainly in the process of rice DNA replication and cell division.It can be used as a candidate gene for rice cold-resistant and drought-resistant breeding.