摘要
以DPPH自由基清除率和蘑菇酪氨酸酶抑制率为评价指标,对不同溶剂提取的川芎提取物、不同极性部位的活性进行筛选;在细胞水平上分别测定提取物对不同刺激物(α-MSH、Forskolin、IBMX)作用下的黑色素含量、细胞内酪氨酸酶活力的影响;DCFH-DA法测定提取物对UV刺激下细胞内ROS的清除作用。结果表明:川芎的50%乙醇水提取物兼具较高的抗氧化和蘑菇酪氨酸酶抑制作用;对三种刺激物作用下的黑色素含量和细胞内酪氨酸酶活性具有很好的抑制作用;能剂量依赖性地减少UV刺激下的ROS含量。川芎提取物以抑制细胞内酪氨酸酶活性、清除ROS双靶点的方式起到美白作用。
The activities of Ligusticum Chuangxiong Hort.(LH)extracted from different solvents and polar sections were investigated with DPPH free radical scavenging rate and mushroom tyrosinase inhibition rate as evaluation indicators.The effects of LH extracts on melanin contents and intracellular tyrosinase activity under various stimuli(α-MSH、Forskolin、IBMX)were detected in cells.The scavenging ability of LH extracts to intracellular ROS induced by UV was estimated by DCFH-DA.The results display that the 50%ethanol-water extracts of LH possess great antioxidant and mushroom tyrosinase inhibitory effects.The LH extracts can achieve good inhibitory effect on melanin contents and intracellular tyrosinase activity stimulated by three stimuli.The LH extracts eliminate ROS induced by UV in a dose-dependent manner.LH extracts has whitening efficacy by dual targets of inhibiting intracellular tyrosinase and scavenging ROS.
作者
王勇恒
梁彦会
孙培冬
WANG Yong-heng;LIANG Yan-hui;SUN Pei-dong(School of Chemical&Material Engineering,Jiangnan University,Wuxi,Jiangsu 214122,China;Key Laboratory of Synthetic and Biological Colloids,Ministry of Education,Jiangnan University,Wuxi,Jiangsu 214122,China;China Association of Fragrance Flavour and Cosmetic Industries,Beijing 100079,China)
出处
《日用化学工业》
CAS
CSCD
北大核心
2020年第4期249-254,共6页
China Surfactant Detergent & Cosmetics
关键词
川芎
美白
双靶点
黑色素
抗氧化
酪氨酸酶
Ligusticum chuanxiong Hort.
whitening
dual-target
melanin
antioxidant
tyrosinase