1-脱氧野尻霉素对高脂饮食肥胖小鼠脂代谢的改善作用

Effect of DNJ on Improving Lipid Metabolism in High-fat Diet-induced Obese Mice

探讨1-脱氧野尻霉素(DNJ)对高脂饮食肥胖小鼠脂代谢的影响及作用机理,为DNJ降脂机理研究提供理论依据。通过喂饲高脂饲料建立小鼠肥胖模型,在持续饲喂高脂饲料的同时灌胃不同剂量的DNJ,40 d后测定实验小鼠一般生理指标,血清及肝脏的相关指标。结果表明,与高脂对照组相比,灌胃8.0 mg/kg bw/d DNJ可显著降低高脂饮食肥胖小鼠的体重、腹腔脂肪系数及肝脂肪含量(P<0.05);显著降低血清中总胆固醇(TC)、甘油三脂(TG)、低密度脂蛋白胆固醇(LDL-C)、游离脂肪酸(FFA)、内脂素水平,显著增加高密度脂蛋白胆固醇(HDL-C)、脂联素(ADP)含量(P<0.05);8.0 mg/kg bw/d DNJ可显著降低肝脏组织中促进脂肪合成的相关酶脂肪合成酶(FAS)、乙酰辅酶A羧化酶(ACC),显著增加分解相关酶酰基辅酶A氧化酶(ACO)、肉毒碱脂酰转移酶Ⅰ(CPT-1)的酶活(P<0.05)。结论:DNJ可能通过控制脂肪因子分泌及脂代谢相关酶活性来抑制脂肪合成,促进脂肪分解,达到调控高脂饮食肥胖小鼠脂代谢的目的。

The effect and mechanisms of DNJ on high-fat diet-induced obese mice were investigated,aiming to provide theoretical basis for the development of health food.Obesity model of mice were established by high fat diet,then all mice were treated with DNJ by oral gavage at different doses for 40 d.Then,the serum-and liver-related indicators were determined.Compared with the high-fat control group,8.0 mg/kg bw/d of DNJ treatment significantly reduced the weight,abdominal fat factor,content of liver fat,and the levels of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)and free fatty acid(FFA),visfatin(P<0.05).8.0 mg/kg bw/d of DNJ treatment also significantly increased content of high density liptein cholesterol(HDL-C),adiponectin(ADP)(P<0.05),reduced the activity of enzymes involved in promote fat synthesis fatty acid synthetasein(FAS)and acetyl Coa carboxylasec(ACC),and significantly increased activity of enzymes in decomposition related Acyl-CoA oxidase(ACO)and carnitine palmitoyl transferase-1(CPT-1)(P<0.05).Conclusion:DNJ could regulate lipid metabolism in obese mice by inhibiting fat synthesis and promote its decomposition through controlling the secretion of adipokine and the activity of enzyme related with lipid metabolism.