宁夏产区不同品种枸杞中总酚含量分析

Analysis of Polyphenols Content in Different Varieties of Lycium barbarum L. in Ningxia Producing Area

借助超声波辅助提取,采用福林酚法对枸杞中总酚含量进行测定。通过优化料液比、提取溶液浓度、提取时间,确定枸杞中总酚提取的最佳条件,即70%乙醇、料液比1∶30(g/mL),提取时间为90 min。同时分析宁夏4个产区4个品种枸杞中总酚含量。结果表明:枸杞中总酚含量在4.53 mg/g^16.07 mg/g之间,其中夏果总酚含量在4.53 mg/g^14.57 mg/g,秋果总酚含量在11.60 mg/g^16.07 mg/g,秋果中总酚含量明显高于夏果,是夏果的1.1~2.6倍。同一品种在不同产区总酚含量存在显著差异。

The total phenolic acid contents in Lycium barbarum L. were determined by the Folin-Ciocalteu method. The ultrasonic-assisted extraction conditions were optimized.The material liquid ratio,extration solution concentration and extration time were optimized by single factor method.The results showed that extration solution was 70 %,the ratio of solid to liquid 1 ∶ 30(g/mL),extration time 90 min. The polyphenols content which thirty Lycium barbarum L. samples in four different varieties of four producing area in Ningxia were analyzed. The results showed that the total phenolic acid contents between 4.53 mg/g and 16.07 mg/g,which the content of summer fruit were 4.53 mg/g-14.57 mg/g and the content of autumn fruit were 11.60 mg/g-16.07 mg/g. The content of autumn fruit was higher and 1.1-2.6 times of summer fruit.There were significant differences in total phenol content of the same variety in different producing areas.