喹诺酮类药物印迹仿生抗体的研制及应用进展

Development and application progress of quinolone imprinted biomimetic antibody

随着人工合成的喹诺酮类抗菌药在动物性食品中残留和环境中蓄积日趋严重,其安全和健康风险促使开发快速且灵敏的检测方法已迫在眉睫。本文介绍了喹诺酮类药物印迹仿生抗体的制备方法,包括本体聚合法、悬浮聚合法、原位聚合法、沉淀聚合法及表面分子印迹法;总结了其作为萃取介质及传感识别元件在肉、蛋、奶、土壤、水体及生物样品检测中的应用;结合分子印迹技术制备工艺及应用的最新研究进展,讨论了该类仿生抗体在制备及识别过程中所面临的印迹容量和效应低、识别模式和传质机理研究不透彻等问题;提出了相应的解决方案,包括新型功能单体的优选及合成、高性能识别模式和制备方法的构建及开发等,为新型印迹仿生抗体的研制及应用提供了参考思路和技术途径。

As the synthetic quinolone antibacterial residues in the animal-derived foods and accumulation in the environment become more and more serious,it is urgent to develop rapid and sensitive monitoring methods to reduce their safety and health risks.This paper introduces the preparation methods of quinolone imprinted biomimetic antibodies,including bulk polymerization,suspension polymerization,in-situ polymerization,precipitation polymerization and surface molecular imprinting,and summarizes its applications in the surveillance of meat,eggs,milk,soil,water and biological samples as the solid-phase adsorbent and the recognition component for sensing detection.Combining with the latest research progress in the preparation process and application of molecular imprinting technology,this paper discusses some problems in preparation and recognition of biomimetic antibodies,such as low imprinting capacity and effect,incomplete research on recognition mode and mass transfer mechanism and so on.Furthermore,we put forward the corresponding solutions of optimizing and synthesizing new functional monomers,constructing and developing high-performance recognition patterns and preparation methods and so on.This will provide the reference idea and technical approach for development and application of novel biomimetic imprinted antibodies.