人参中腐霉利农药残留量测定研究

Determination of Procymidone Pesticide Residues in Ginseng

目的建立气相色谱—质谱联用法(GC—MS)测定人参中腐霉利的方法。方法样品用乙腈提取,通过预先装有净化材料的分散固相萃取净化管中,涡旋使充分混匀,再置振荡器上剧烈振荡使净化完全,然后经气相色谱-质谱法分析腐霉利的残留量,以外标法定量。结果腐霉利(速克灵)质量浓度在0.008~0.2μg范围内时,线性关系良好,相关系数r为0.99997,检出限为0.005μg/mL。称取人参样品平行6份,分别加入一定量腐霉利标准溶液(0.02μg/mL),按方法步骤处理测定,计算回收率在78.83%~96.21%范围内,相对标准偏差为3.96%。结论该方法可适用于人参中腐霉利残留量的检测。

Objective To establish a method for the determination of procymidone in ginseng by gas chromatography-mass spectrometry(GC-MS).Methods Samples were extracted with acetonitrile,through the dispersed solid phase extraction purification pipe which was provided with purification materials in advance,vortex to mix well,placing the oscillator to vibrate violently to make the purification complete,then the residual amount of procymidone was analyzed by gas chromatographymass spectrometry,quantification by external standard method.Results When the mass concentration of procymidone(sucrin)was in the range of 0.008μg^0.2μg,good linear relationship,the correlation coefficient(r)was 0.99997,the detection limit was 0.005μg/m L.Ginseng samples were measured in 6 parallel samples,a certain amount of standard solution of procymidone(0.02μg/mL)was added respectively,according to the method step processing determination,the calculated recovery ranged from 78.83%to 96.21%,the relative standard deviation was 3.96%.Conclusion The method can be applied to the determination of residual procymidone in ginseng.