组方优化的阳和胶囊对碘乙酸钠诱导大鼠骨关节炎的作用研究

Effect of Yanghe capsule with optimized formula on osteoarthritis induced by sodium iodoacetate in rats

目的探讨组方优化的阳和胶囊对碘乙酸钠诱导的大鼠骨关节炎的治疗作用及其机制研究。方法采用大鼠膝关节腔内注射4%碘乙酸钠溶液0.1 ml,连续注射7天,建立大鼠骨关节炎模型。于造模后分别灌胃给予组方优化的阳和胶囊高、中、低剂量及阳性对照双醋瑞因胶囊(8.0mg/kg),每日1次,连续给药4周。实验期间每周测定大鼠膝关节肿胀度。实验结束后测定大鼠膝关节灌洗液中NO、PGE-2含量,血清中IL-1β、TNF-α含量,及软骨组织中MMP-1、MMP-13含量。结果实验期间模型组大鼠膝关节显著肿胀(P<0.01),各给药组与模型组相比均有不同程度缓解。组方优化的阳和胶囊高、中剂量组能够显著降低膝关节灌洗液中NO、PGE-2含量(P<0.01)、显著降低血清中IL-1β、TNF-α含量(P<0.01)明显降低软骨组织中MMP-1、MMP-13含量(P<0.05)。结论组方优化的阳和胶囊对碘乙酸钠诱导的大鼠骨关节炎具有治疗作用,其机制可能与降低膝关节灌洗液及血清中NO、PGE-2、IL-1β、TNF-α水平及软骨组织中MMP-1、MMP-13含量有关。

Objective To explore the therapeutic effect and mechanism of Yanghe capsule with optimized formula on osteoarthritis induced by sodium iodoacetate.Methods A rat model of osteoarthritis was established by injecting 0.1 ml of 4%sodium iodoacetate solution into the knee joint for 7 days.After the establishment of the model,Yanghe capsule with high,medium and low dose and positive control(8.0 mg/kg)was administrated by gavage once a day for 4 weeks.During the experiment,the swelling degree of the knee joint was measured every week.At the end of the experiment,the contents of NO,PGE-2,IL-1β,TNF-α,MMP-1 and MMP-13 were measured.Results During the experiment,the knee joint swelling of the model group was significant(P<0.01),and each administration group was relieved in different degrees compared with the model group.The content of NO,PGE-2,IL-1β,TNF-α,MMP-1 and MMP-13 were significantly reduced in the high and middle dose groups of Yanghe capsule optimized by the formula(P<0.01).Conclusion Yanghe capsule has therapeutic effect on osteoarthritis in rats induced by sodium iodoacetate.The mechanism may be related to the decrease of NO,PGE-2,IL-1β,TNF-αlevels in knee joint lavage fluid and serum,and the contents of MMP-1 and MMP-13 in cartilage.