羟氯喹对狼疮小鼠脾淋巴细胞的影响

Effect of hydroxychloroquine on spleen lymphocytes in lupus mice

目的观察羟氯喹对系统性红斑狼疮小鼠(TC小鼠)外周免疫器官(脾)淋巴细胞分布的影响。方法将30周龄的雌性TC小鼠随机分配,实验组经灌胃给予羟氯喹治疗处理[10 mg/(kg·d)],对照组经灌胃给予生理盐水处理[10 mg/(kg·d)],共处理5周。用酶联免疫吸附试验(ELISA)检测小鼠外周血抗dsDNA抗体滴度水平,苏木精-伊红(HE)染色法检测肾组织病理学改变,流式细胞术检测小鼠脾淋巴细胞比例。结果与对照组相比,实验组小鼠肾组织病理学评分显著低于对照组(P<0.05);实验组脾B淋巴细胞和生发中心B淋巴细胞比例显著低于对照组(P<0.05);实验组脾调节性T淋巴细胞(Treg)比例显著高于对照组(P<0.05);两组小鼠抗dsDNA抗体水平变化及Th17淋巴细胞比例变化无明显差异(P>0.05)。结论羟氯喹对狼疮小鼠外周免疫器官淋巴细胞的免疫应答具有调节作用并且可缓解TC小鼠肾组织病理损伤。

Objective To observe the effect of hydroxychloroquine on lymphocyte distribution in the peripheral immune organs(spleen)of systemic lupus erythematosus mice(TC mice).Methods Thirty-week-old female TC mice were randomly assigned to two groups:the experimental group was treated with hydroxychloroquine[10 mg/(kg·d)]and the control group was treated with normal saline[10 mg/(kg·d)]for 5 weeks.Enzyme-linked immunosorbent assay was used to detect the titer of anti-dsDNA antibody in the peripheral blood of mice,Hematoxylin-eosin staining was used to detect histopathological changes in kidneys,and flow cytometry was used to detect the proportion of lymphocytes in the spleen of mice.Results The histopathological score of kidneys in the experimental group was significantly lower than that in the control group(P<0.05).The proportions of splenic B lymphocytes and germinal center B lymphocytes in the experimental group were significantly lower than that in the control group(P<0.05).The proportion of splenic regulatory T lymphocyte(Treg)in the experimental group was significantly higher than that in the control group(P<0.05).There were no significant difference in the levels of anti-dsDNA antibody and the T helper cell 17(Th17)lymphocyte proportions between groups(P>0.05).Conclusions Hydroxychloroquine has a regulatory effect on the immune response of lymphocytes in peripheral immune organs of lupus mice and alleviated the pathological damage in kidney tissues of TC mice.