心肌铁过载兔模型的制作及铁沉积规律的研究

Establishment of a rabbit model of cardiac iron overload and its relationship with iron deposition

目的探讨心肌铁沉积动物模型的制作方法及心脏、肝之间铁沉积规律的关系。方法选取40只雄性新西兰白兔随机分成实验组32只,对照组8只。每周一实验组白兔经皮下注射右旋糖酐铁200 mg/kg;于每周日随机处死2只白兔,每间隔1周的周日随机处死1只对照组白兔;取出部分心脏、肝组织,用生理盐水反复冲洗至排尽心脏、肝组织内残留血液,置于电热恒温鼓风干燥箱(温度65℃)烘干至重量不再变化。烘干后的心脏、肝组织用火焰原子吸收分光光度计测量心、肝铁浓度(CIC、LIC)并将部分心、肝组织固定后行HE染色及普鲁士蓝染色。结果随着注射铁剂的增多,病理结果显示实验组家兔心肌普鲁士蓝染色铁颗粒增多,CIC逐渐增高(范围0.20~2.34 mg/g干重,中位数1.24 mg/g干重)。CIC与注射铁总量之间中度相关(r=0.698,P<0.05,P<0.001),CIC与血清铁之间中度相关(r=0.415,P<0.05,P=0.022)。CIC与LIC之间高度相关(r=0.712,P<0.05,P<0.001)。结论通过皮下注射剂量为200 mg/kg的右旋糖酐铁,可以建立心肌铁过载兔模型,心脏、肝之间铁沉积没有相关性。

Objective To establish an animal model of heart iron overload and investigate the relationship between iron deposition in the heart and liver.Methods Forty rabbits were randomly divided into the experimental group(n=32)or control group(n=8).The experimental group was injected subcutaneously with 200 mg/kg of iron dextran every Monday.Two rabbits were randomly euthanized every Sunday.The heart and liver tissues were removed,rinsed with normal saline repeatedly,and placed in an oven at 65℃to dry until the weight was unchanged.A flame atomic absorption spectrophotometer was used to measure the iron concentration of hearts and livers(CIC,LIC).Hematoxylin and eosin staining and Prussian blue staining were performed.Results With the increase of iron injection,pathological result showed that Prussian blue staining of iron particles increased in the experimental group,and that the CIC increased gradually(0.20-2.34 mg/g dry weight,median 1.24 mg/g dry weight).CIC was moderately correlated with total injected iron(r=0.698,P<0.05,P<0.001),and CIC was moderately correlated with serum iron(r=0.415,P<0.05,P=0.022).CIC was highly correlated with LIC(r=0.712,P<0.05,P<0.001).Conclusions A rabbit model of cardiac iron overload was established by the subcutaneous injection of dextran iron at a dose of 200 mg/kg,and there was no correlation between heart and liver iron deposition.