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基于HPLC法对加入厚朴后广藿香水溶性物质的含量影响研究

Effect on the content of water-soluble substances in patchouli after adding magnolia officinalis by HPLC
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摘要 建立高效液相色谱法测后对其广藿香加入厚朴两种水溶性成分香草酸和原儿茶酸的含量影响。采用蒸馏水为溶剂的加热回流法进行提取,广藿香与厚朴配伍后原儿茶酸含量是配伍前的1.6倍,香草酸含量升高明显,配伍后香草酸含量约是配伍前的15.9倍。采用HPLC法对广藿香加入厚朴前后的两种水溶性成分(香草酸,原儿茶素酸)进行测定,该方法科学易行、操作简单、结果准确。 The effect of adding high-performance liquid chromatography on the content of vanillic acid and Protocatechuic acid in the two water-soluble components of magnolia officinalis was established.The extract was extracted by distilled water as the solvent.The content of protocatechuic acid in the combination of patchouli and magnolia was 1.6 times that before the compatibility,and the content of vanillic acid was obviously increased.The content of vanillic acid after compatibility was about 15.9 times that before the compatibility.HPLC method was applied to the determination of two water-soluble components(vanillic acid,protocatechuic acid)before and after adding Magnolia sinensis.The method was scientific,easy to operate and accurate.
作者 齐乐辉 李嘉欣 孟鑫 田园 许树军 QI Le-hui;LI Jia-xin;MENG Xin;TIAN Yuan;XU Shu-jun(Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处 《化学工程师》 CAS 2020年第4期19-21,8,共4页 Chemical Engineer
基金 黑龙江中医药大学科研基金项目(面上项目:201719) 黑龙江省大学生创新创业训练计划项目(国家级创新训练项目:201810228008)。
关键词 广藿香:HPLC 厚朴 香草酸 原儿茶素酸 patchouli HPLC magnolia vanilic acid protocatechuic acid
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