蜂王浆对H2O2所致NIH-3T3细胞损伤的修复作用及机理

Repair effects and mechanism of royal jelly on NIH-3T3 cellular damage caused by H2O2

目的观察蜂王浆(RJ)对H2O2诱导的小鼠胚胎成纤维细胞(NIH-3T3)损伤的修复作用,并探讨其可能的分子机制。方法以20μg·mL-1 H2O2处理NIH-3T3细胞建立细胞损伤模型,用不同浓度的蜂王浆处理模型细胞,检测各组细胞的增殖活性、β-半乳糖苷酶(SA-β-Gal)染色阳性细胞率、细胞凋亡情况以及细胞衰老相关基因的变化。结果 NIH-3T3细胞经H2O2处理后增殖能力下降,SA-β-Gal阳性率升高,p16、p21、p53mRNA过高表达;RJ处理后上述指标均有所改善。结论 RJ能延缓H2O2所致的细胞损伤和衰老。其机制可能和RJ在翻译前的环节抑制p16、p21、p53基因的表达并通过p16-cyclinD/CDK-RB和p53/P21信号通路调控细胞周期及凋亡程序有关。

OBJECTIVE To study the repair effects of royal jelly(RJ) on mouse embryonic fibroblasts(NIH-3 T3) cellular damage caused by H2O2 and to explore its molecular mechanism.METHODS NIH-3 T3 cells were treated with 20 μg·mL-1 H2O2 to establish cellular damage model.The model cells were treated with RJ of different concentrations.The each group′s cell proliferation activity,positive cell rate stained with β-galactosidase,apoptosis,and expression level of p16,p21 and p53 genes were tested.RESULTS The proliferation ability of NIH-3 T3 cells was decreased,and the positive rate of SA-β-gal,apoptosis,and expression of p16,p21,p53 genes were increased after H2O2 treated.RJ can improve these indicators.CONCLUSION RJ can improve cellular damage and senescence induced by H2O2.The mechanism is related to inhibiting expression of p16,p21 and p53 genes before the translation process,working through the p16-cyclinD/CDK-RB and p53/p21 signaling pathway.