温度、盐度和照度对菌藻联合体氨氮吸收的影响

Effects of Temperature,Salinity and Light on NH4+-N Absorption of Bacteria-Microalgae Consortia

【目的】探究温度、盐度和照度对侧孢短芽孢杆菌(Brevibacillus laterosporus)和威氏海链藻(Thalassiosira weissflogii)联合体系氨氮吸收的影响。【方法】利用稳定同位素示踪技术测定侧孢芽孢杆菌和威氏海链藻对氨氮的吸收速率,分析不同环境因子水平下侧孢短芽孢杆菌和威氏海链藻联合体系对氨氮吸收的规律。【结果】单因子实验结果显示温度、盐度和照度对菌藻联合体氨氮吸收速率影响显著(P<0.05),当温度25~35℃,盐度20和照度8000 lx时菌藻联合体氨氮吸收速率较高;正交实验结果显示,温度25℃,盐度25和光照4000 lx是菌藻联合体氨氮吸收的最优组合条件,盐度是影响其吸收速率的主要因子,其次是温度和照度;威氏海链藻在菌藻联合体系氨氮吸收中占主要贡献。【结论】侧孢短芽孢杆菌和威氏海链藻所构建的联合体系可在高温、高盐和强光环境下实现对对虾池塘中过量氨氮的有效吸收,在改善我国华南地区对虾养殖池塘的水质方面具有广阔的应用前景。

【Objective】Effects of temperature,salinity,and light on NH4+-N uptake by Brevibacillus laterosporus and Thalassiosira weissflogii were studied.【Method】Stable isotope tracer technology was used to measure the absorption rate of NH4+-N by B.laterosporus and T.weissflogii,which use to analyze the NH4+-N absorption by the combined system under different environmental factor levels.【Result】Temperature,salinity and illumination had significant effects on the NH4+-N absorption rate of bacteria-algae consortia(P<0.05).The rate of NH4+-N absorption of the bacteria-algae consortia was higher from 25-35℃,salinity 20,and 8000 lx.The best conditions for the absorption of NH4+-N by the bacteria-algae consortia were 25℃,salinity 25,and 4000 lx.The microalgae played a major role in this system.【Conclusion】The bacteria-algae consortia in our study can achieve effective absorption of excess NH4+-N in shrimp ponds under high temperature,high salinity,and strong light environment.Therefore,it may have a broad application prospects in improving the water quality of shrimp culture ponds in Southern China.