等边浅蛤肉酶解产物超滤组分免疫调节作用

Immunomodulatory Effects of the Ultrafiltration Fractions of Enzymatic Hydrolysates from the Edible Part of Gomphina aequilatera

【目的】探讨等边浅蛤(Gomphina aequilatera)肉的基本营养成分、蛋白质氨基酸组成及中性蛋白酶酶解产物超滤组分的免疫活性。【方法】采用中性蛋白酶酶解等边浅蛤肉,利用3ku超滤膜对等边浅蛤肉酶解产物进行超滤分离,通过细胞实验和动物实验评价小于3 ku和大于3 ku两个超滤组分的免疫调节作用。【结果】等边浅蛤肉具高蛋白低脂肪特点,其氨基酸种类和含量丰富。细胞实验表明,<3 ku组分的细胞相对增殖率及吞噬中性红能力均显著高于阳性对照组(P<0.05),其中质量浓度为250μg/mL时细胞相对增殖率高达126.22%;动物实验表明,与空白对照组相比,<3 ku组分能够极显著提高迟发型变态反应(DTH)程度、半数溶血值(HC50)、抗体生成细胞数、吞噬指数及NK细胞活性(P<0.01)。细胞实验和动物实验均表明<3 ku组分的免疫调节作用优于>3 ku组分。【结论】等边浅蛤肉酶解产物的<3 ku超滤组分具有一定的免疫调节作用。

【Objective】This study investigated the essential nutrients,amino acid composition of protein and the immunoactivity of the ultrafiltrated enzymatic hydrolysis products of Gomphina aequilatera meat.【Methods】A 3 ku ultrafiltration membrane was used to separate the neutral protease hydrolyzate of the Gomphina aequilatera,and then cell and in vivo experiments were conducted to test the immunomodulatory effects of ultrafiltration components.【Results】Gomphina aequilatera meat has high contents of protein and low fat level.Its amino acid species and content are rich.Result of cell experiments showed that the relative proliferation rate and the ability to devour neutral red of ultrafiltration component with MW<3 ku were higher than those of the positive control group(P<0.05).When the mass concentration is 250μg/mL the relative proliferation rate was as high as 126.2%.In vivo data showed that the degree of delayed allergic reaction(DTH),hemolytic value(HC50),the number of antibody-producing cells,phagocytic index and NK cell activity were higher than these of controls(P<0.01).Both cell culture and in vivo results showed that the immunomodulatory effect of the ultrafiltration component with molecular weight<3 ku was better than that of>3 ku.【Conclusion】The<3 ku hydrolyzate fraction from the of Gomphina aequilatera has immunomodulatory effects.