不同产地雷公藤中4种有效成分的含量测定

Determination of Four Effective Constituents in Tripterygium wilfordii Hook.f.from Different Producing Areas

[目的]建立同时测定雷公藤中4种有效成分含量的方法,并研究不同产地雷公藤中4种有效成分的含量差异。[方法]采用Thermo Hypersil GOLD Phenyl Dim(250 mm×4.6 mm,5μm)色谱柱;以乙腈-0.1%磷酸-0.2%三乙胺溶液为流动相,梯度洗脱;检测波长:0~65 min为225 nm,65 min后为210 nm;流速0.75 mL/min;柱温25℃;进样量10μL。[结果]4种有效成分均有较好的分离度,方法精密度和重复性RSD均小于3.7%,待测样品于室温下24 h内能保持稳定,4种有效成分均有良好的线性关系,加样回收率分别为雷公藤甲素86.72%、雷公藤内酯酮107.51%、雷公藤红素76.94%、雷公藤酯甲84.38%。[结论]该方法精密度和重现性良好,可同时测定雷公藤中4种有效成分的含量,可为雷公藤的质量控制提供科学依据。

[Objective]To establish a method for simultaneous determination of four effective ingredients of Tripterygium wilfordii,and to study the difference of these four effective constituents from different producing areas.[Method]Samples were analyzed on a Thermo Hypersil GOLD Phenyl Dim chromatographic column(250 mm×4.6 mm,5μm),and eluted with the mobile phase of acetonitrile-0.1%phosphorus-0.2%triethylamine solution with a linear gradient mode,the detection wavelengths were set at 225 nm for 0-65 min and 210 nm for 65 min,the flow rate of the mobile phase was 0.75 mL/min with the column temperature of 25℃,and the determined volume was 10μL.[Result]These four effective constituents were well separated,the RSD of precision and repeatability both less than 3.7%,and the samples remained stable for 24 hours at room temperature.Meanwhile these four effective constituents exhibited a good linearity,and the recoveries of triptolide,triptonide,celastrol and wilforlide was 86.72%,107.51%,76.94%and 84.38%,respectively.[Conclusion]The method is accurate and repeatable,and it can be used for the determination of the four components in Tripterygium wilfordii.