枇杷花发育进程中氨基酸和碳水化合物代谢的变化

Studies on the Amino Acid Metabolism and Carbohydrate Metabolism Variation During Flower Development in Eriobotrya japonica

为研究影响枇杷(Eriobotrya japonica Lindl.‘Ninghaibai’)花的发育相关代谢机制,以花芽生理分化期、花芽形态分化期、花穗发育期、小花发育期和开花期这5个阶段的当年生春梢上顶芽、花芽或花穗为试验材料,采用GC–MS技术检测其代谢物质,并将5个阶段的差异代谢物质进行统计和归类,筛选出了相关性最高的代谢通路。此外,测定其可溶性糖、可溶性蛋白、淀粉含量以及C/N比,分析各营养物质在花不同发育阶段的变化。在整个发育进程中共检测出430种代谢物,氨基酸代谢和碳水化合物代谢是两个变化最显著的代谢通路,而小花发育期是代谢最为旺盛的阶段。可溶性糖、可溶性蛋白、淀粉含量以及C/N比的变化趋势与代谢组表型结果一致,C/N比是决定花发育进程的重要因素之一。

In order to study the metabolic mechanism related to flower development in Eriobotrya japonica,GC–MS was used as the detection technique. The metabolites in five different developmental processes were detected at physiological differentiation stage,morphological differentiation stage,inflorescence emergence and expansion stage,single flower development stage and blossoming stage. Multivariate statistical analysis was carried out for all the samples,and then the metabolites were statistically classified and the most relevant metabolic pathways were screened out. In this research,the metabolic phenotypes of different flower developmental stages were summarized and analyzed. The soluble sugar,soluble proteins and starch content as well as C/N ratio variation during the five stages were also detected. A total of 430 metabolites were obtained. Amino acid and carbohydrate metabolism were the two most significant metabolic pathways related to flower development,and the metabolism was more vigorous in single flower development stage than the other stages. The soluble sugar,soluble proteins, starch content and C/N ratio were consistent with the phenotypic results of the metabolome. C/N ratio is one of the important factors determining the development process of flowers.