血清miRNA-144与冠状动脉粥样硬化严重程度的相关性

Correlation between the miRNA-144 in serum and the severity of coronary atherosclerosis

目的探讨冠心病患者血清miRNA-144表达水平与冠状动脉粥样硬化严重程度的相关性。方法选取冠心病患者67例(冠心病组)和同期来医院体检中心进行体检无冠心病体检者30例(对照组),收集基本临床和血生化资料,使用PCR法检测两组样本的血清中miRNA-144的表达水平,使用Gensini评分法评价冠状动脉病变严重程度,进行单因素和多因素分析。结果冠心病组患者体内血清miRNA-144的表达水平较对照组显著升高(P<0.01),其中急性心肌梗死亚组患者的miRNA-144的表达较对照组变化量最明显。Gensini评分与miRNA-144的表达量呈明显的正相关(r=0.69,P<0.01),受试者工作特征曲线(receiver operating curve,ROC)分析结果显示曲线下面积(area under curve,AUC)为0.9888(P<0.01),灵敏度和特异度分别为98%和98%。结论冠心病患者血清中miRNA-144表达水平升高,且其表达水平与冠状动脉粥样硬化严重程度呈正相关。

AIM To study the difference of serum miRNA-144 expression between patients with coronary heart disease and healthy people,and to explore its correlation with the severity of coronary atherosclerosis.METHODS 67 patients with coronary heart disease and 30 healthy people were selected.The basic clinical and blood biochemical data of the two groups were collected.Real-time quantitative PCR was used to detect the expression level of miRNA-144 in the serum of the sample,and the expression difference was analyzed.The Gensini score was used to evaluate the severity of coronary lesions and to analyze the associations.RESULTS The expression of miRNA-144 in serum of patients with coronary heart disease was significantly higher than that of the control group,and the difference was statistically significant(p < 0.01).Among them,the expression of miRNA-144 was the most obvious in patients with acute myocardial infarction,which was about 2.6 times of the control group.The Gensini score was positively correlated with the expression of miRNA-144(r = 0.69,P<0.01).The ROC curve analysis showed that the AUC was 0.9888(P<0.01),and the sensitivity and specificitly were 98% and98%,respectively.CONCLUSION The expression of miRNA-144 in serum of patients with coronary heart disease increases,and its expression is positively correlated with the severity of coronary atherosclerosis.