细胞分裂周期7蛋白在肝癌组织的表达及其对肝癌细胞增殖和侵袭的影响

Expression of cell division cycle 7 protein in hepatocellular carcinoma tissues and its effect on proliferation and invasion of hepatocellular carcinoma cells

目的检测细胞分裂周期7(CDC7)蛋白在人肝癌组织的表达,探讨CDC7过表达对肝癌细胞增殖和侵袭的影响。方法实时定量聚合酶链式反应(Real-time PCR)检测CDC7 mRNA在肝癌组织和癌旁组织的表达,检测CDC7 mRNA在肝癌细胞系和正常肝脏细胞中的表达。Western blot检测CDC7蛋白在肝癌组织和癌旁组织中的表达,检测CDC7蛋白在肝癌细胞系和正常肝脏细胞中的表达水平。利用慢病毒载体构建稳定过表达CDC7的肝癌HCCLM3和SMMC 7721细胞株。细胞克隆实验和CCK-8法检测CDC7过表达对肝癌细胞增殖的影响。Transwell实验和划痕实验检测CDC7过表达对肝癌细胞侵袭迁移的影响。结果与癌旁组织相比,CDC7 mRNA和蛋白在肝癌组织中表达水平显著升高(P<0.001);与正常肝细胞相比,肝癌细胞系的CDC7 mRNA和蛋白表达水平显著增高(P<0.001);CCK-8法、细胞克隆实验说明CDC7过表达会明显增强肝癌细胞的增殖能力;划痕实验、Transwell实验说明CDC7过表达会明显提高肝癌细胞的侵袭能力。结论CDC7蛋白在人肝癌组织高表达,其过表达促进肝癌细胞的增殖和侵袭能力。

Objective The objectives of this study were to investigate the expression of cell division cycle 7 protein(CDC7)in hepatocellular carcinoma(HCC)tissues,and to explore the effect of CDC7 overexpressing on proliferation and invasion of hepatocellular carcinoma cells.Methods Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression of CDC7 mRNA in liver cancer tissues and adjacent tissues,and the expression of CDC7 mRNA in liver tumor cell lines and normal liver cells.Western blot was used to detect the expression of CDC7 protein in liver cancer tissues and adjacent tissues,and to detect the expression of CDC7 protein in liver tumor cell lines and normal liver cells.Lentivirus vectors were used to construct stable overexpressing CDC7 hepatocellular carcinoma HCCLM3 and SMMC 7721 cell lines.Cell clone and cell counting kit-8(CCK-8)experiments were used to detect the effect of CDC7 overexpression on the proliferation of HCC cells.Scratch and Transwell assays were used to detect the effect of CDC7 overexpression on the invasion and migration of HCC cells.Results Compared with adjacent tissues,the expression of CDC7 at the levels of mRNA and protein was significantly increased in HCC tissues(P<0.001);compared with normal liver cells,the expression levels of CDC7 at the levels of mRNA and protein were significantly increased in liver cancer cell lines(P<0.001);CCK-8 experiments and cell cloning experiments showed that overexpression CDC7 significantly enhanced the proliferative ability of HCC cells;Scratch and Transwell experiments showed that overexpression CDC7 significantly improved the invasion ability of HCC cells.Conclusion CDC7 protein is highly expressed in human HCC tissues,and its overexpression promotes the proliferative and invasive capability of HCC cells.