嗜酸乳杆菌GIM1.208产β-葡萄糖苷酶培养基及发酵条件优化

Optimization of medium and fermentation conditions forβ-glucosidase production by Lactobacillus acidophilus GIM1.208

以嗜酸乳杆菌(Lactobacillus acidophilus)GIM1.208为研究对象,通过单因素、正交试验和响应面法优化其产β-葡萄糖苷酶的培养基及发酵条件。结果表明,嗜酸乳杆菌GIM1.208发酵产β-葡萄糖苷酶的最佳产酶条件为葡萄糖添加量3.0%,羧甲基纤维素钠添加量0.4%,初始pH值5.5,料液比1∶4(g∶mL)、发酵温度31℃,发酵时间24 h,接种量2.6%。在此优化条件下,β-葡萄糖苷酶活力为16.80 U/mL,是优化前的3.90倍。

Using Lactobacillus acidophilus GIM1.208 as the research object,the medium and fermentation conditions forβ-glucosidase production were optimized by single factor,orthogonal tests and response surface methodology.The results showed that the optimum production conditions ofβ-glucosidase by L.acidophilus GIM 1.208 were glucose 3.0%,sodium carboxymethylcellulose 0.4%,initial pH 5.5,material to liquid ratio 1∶4(g∶ml),fermentation temperature 31℃,fermentation time 24 h,and inoculum 2.6%.Under the optimal conditions,theβ-glucosidase activity was 16.80 U/ml,which was 3.90 times that of before optimization.