光质对不同家系青钱柳叶酚类物质积累及抗氧化活性的影响

Effects of light quality on accumulation of phenolic compounds and antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves from different families

【目的】光质对植物体内酚类物质的积累有着重要的影响。青钱柳作为我国特有的单种属乔木,功能多样,具有材用、药用、观赏价值。通过研究光质处理对不同家系青钱柳叶酚类物质积累及抗氧化活性的影响,为青钱柳苗期培育过程中的环境选择和进一步开发保健产品提供理论依据。【方法】在人工气候室内可控温湿度的条件下对不同家系(广西金钟山7号、广西金钟山6号、四川沐川31号、浙江安吉1号)青钱柳当年生实生苗进行白光、蓝光、绿光和红光4种光质处理,测定其叶片生物量、酚类物质含量及单株产量和体外抗氧化活性[1,1-二苯基-2-三硝基苯肼(DPPH)、自由基清除能力和铁离子还原能力(FRAP)],并分析了抗氧化活性与酚类物质含量的相关性。【结果】除家系对总黄酮含量和单株产量无显著影响外,光质和家系及其交互作用对叶生物量、酚类物质含量和单株产量及抗氧化活性均有显著影响。总体来讲,白光处理下的青钱柳叶生物量最大,酚类物质含量、单株产量及抗氧化活性却最低;蓝光和绿光处理下的青钱柳叶具有较高的酚类含量以及抗氧化活性,但绿光处理下的叶生物量最小。因此,蓝光处理对青钱柳叶酚类物质的积累及其抗氧化能力的提高更有利。从家系层面看,安吉1号家系青钱柳的叶生物量最大,而金钟山6号家系青钱柳的叶酚类含量、酚类单株产量及抗氧化活性最高。相关性分析表明,青钱柳叶总酚及酚酸类物质含量与抗氧化活性呈显著相关(P<0.05)。【结论】光质的调控显著影响不同家系青钱柳叶酚类物质积累和抗氧化活性,蓝光处理下的金钟山6号青钱柳叶的酚类物质积累多且抗氧化能力强。研究结果可为青钱柳保健品的开发及人工林定向培育过程中的光照管理提供参考。

【Objective】Light quality has a certain effect on the accumulation of phenolic compounds in plants. Cyclocarya paliurus(Batal.) Iljinskaja is a unique species in China, it is widely used as a material for wood, medicines and ornamentation. The objective of the present study was to investigate the effect of light quality on the accumulation of phenolic compounds in C. paliurus(Batal.) Iljinskaja leaves from different families and their antioxidant activity and thereby provide a basis for optimizing the light environment during seedling development and further development of C. paliurus(Batal.) Iljinskaja healthcare products.【Method】Current-year seedlings of C. paliurus(Batal.) Iljinskaja from four families(Guangxi Jinzhongshan No. 7, Guangxi Jinzhongshan No. 6, Sichuan Muchuan No. 31 and Zhejiang Anji No. 1) were treated with four light quality groups(white light, blue light, green light and red light) with controlled temperature and humidity in an artificial climate chamber. Leaf biomass, phenolic content and yield in C. paliurus(Batal.) Iljinskaja leaves as well as phenolic antioxidant activity [DPPH(1, 1-diphenyl-2-picrylhydrazyl) radical-scavenging ability and FRAP(ferric reducing power assay)] in vitro were measured, and the correlation between phenolic content and antioxidant activity was analyzed.【Result】Light quality and family and their interaction had significant effects on leaf biomass, phenolic content and yield per plant and antioxidant activity. However, there were no significant effects of family on the total flavonoid content and yield per plant. In general, white light treatment resulted in the highest value of leaf biomass but the lowest value of phenolic content and yield per plant and antioxidant activity. The seedlings treated with blue light and green light showed higher content of phenolics and antioxidant activity, but green light treatment resulted in the lowest value of leaf biomass. Therefore, blue light treatment was more beneficial in improving the accumulation of phenolics and their antioxidant capacity in C. paliurus(Batal.) Iljinskaja leaves. At the family’s level, the leaf biomass of C. paliurus(Batal.) Iljinskaja from Anji No. 1 was the highest. However, the highest values of phenolic content and yield per plant and their antioxidant capacity were observed in Jinzhongshan No. 6. The correlation analysis showed that the content of total phenolics and phenolic acids significantly correlated with the antioxidant activity(P < 0.05). 【Conclusion】Regulation of light quality significantly affected the accumulation of phenolics and their antioxidant activity in the leaves of C. paliurus(Batal.) Iljinskaja belonging to the different families. Leaves of C. paliurus(Batal.) Iljinskaja from Jinzhongshan No. 6 treated with blue light accumulated more phenolics and thereby exhibited higher antioxidant ability. The results provide a reference for the development of C. paliurus(Batal.) Iljinskaja healthcare products and light management for directional cultivation in C. paliurus(Batal.) Iljinskaja plantations.