植物模式识别受体FLS2的研究进展

The progress in plant pattern recognition receptor FLS2

植物通过模式识别受体识别病原微生物保守的分子,开启第一层次免疫屏障,以此实现对各种微生物的非寄主抗性和产生基础防御。第一个被鉴定的植物模式识别受体是识别细菌鞭毛蛋白的拟南芥FLS2,围绕FLS2的大量研究为其他模式识别受体的研究提供了范例,促进了植物免疫理论的建立和发展。通过介绍FLS2的发现过程、命名过程的插曲、结构与功能、激活步骤与相关元件、调控的分子机制、FLS2与病原微生物效应因子的相互作用以及FLS2在被子植物中的系统发生关系,对FLS2研究中显现的蛋白污染和C末端标签问题进行了分析,并介绍了依靠遗传转化植物模式识别受体基因,培育广谱耐久抗病植物的前景。

Plants rely on pattern recognition receptors(PRRs) to perceive conserved signatures of pathogenic microorganisms. This recognition triggers the first layer of immunity, which consists of non-host resistance against various microorganisms and generates basal resistance. Arabidopsis FLS2 is the first PRR identified in plants that recognizes bacterial flagellin. A large number of studies on FLS2 provide examples and basis for future studies of other PRRs, and they promote the establishment and development of the plant immune theory. In the present study, we reviewed the discovery and naming process of FLS2, its structure and function, the process of activation, related components, mechanism of regulation, interaction with effectors, and phylogeny in angiosperms. We also analyzed the technical problems in protein contamination and C-terminal tags accompanying FLS2 research, and we introduced the prospect of breeding plants with broad-spectrum and durable resistance to disease by genetic transformation with plant pattern recognition receptor genes.