光强和温度对圆紫菜叶状体体细胞发育分化的影响

The effects of light intensity and temperature on the development and differentiation of single somatic cells isolated from the gametophytic blades in Pyropia suborbiculata(Bangiales,Rhodophyta)

以日龄60 d的圆紫菜(Pyropia suborbiculata)野生型品系(PS-WT)和突变型品系(PS-L)的叶状体为实验材料,分别酶解获得它们的离体单细胞,观察后者在液体培养基中的发育形态,并探究光强和温度对离体单细胞发育分化的影响。结果表明,圆紫菜叶状体的离体单细胞可发育成不同类型的再生体:正常苗、畸形苗、细胞团和性细胞团等。在光强为10~80μmol photons/(m^2·s)范围内,2个品系离体单细胞的再生体中正常苗、细胞团和丝状体的百分率均不存在显著性差异,适宜的高光[40~60μmol photons/(m^2·s)]可促进再生体单孢子的放散。温度在18~24℃范围内,2个品系离体单细胞的再生体中正常苗、畸形苗的百分率不存在显著性差异;但高温(27~30℃)会抑制正常苗的形成,以及促进畸形苗的形成和再生体单孢子的放散。

The 60-day-old blades of wild-type strain(PS-WT)and mutant strain(PS-L)of Pyropia suborbiculatain were used as experimental materials to obtain their isolated cells by enzymatic hydrolysis,and then observe the development and morphology of isolated cells in submerged culture.Meanwhile,we also investigated the effects of temperature and light intensity on the development and differentiation of isolated cells.The results showed that the isolated cells could develop into different types of regenerative plants:normal blades,abnormal blades,cell-masses and sexual cell-masses.In the range of 10-80μmol photons/(m^2·s),there was no significant difference in the percentage of normal blades,cell-masses and conchocelis of the two strains;appropriate light intensity[40-60μmol photons/(m^2·s)]could promote the release of monospores of regenerative plants.In range of 18-24℃,the proportion of normal blades and abnormal blades of two strains had no significant difference.However,high temperature(27-30℃)could inhibit the formation of normal blades,promote the formation of abnormal blades and the release of monospores the of regenerative plants.