烟草丙酮酸脱羧酶的生物信息学分析

Bioinformatics Analysis of Tobacco Pyruvate Decarboxylase

为进一步了解烟草丙酮酸脱羧酶的特性及功能,采用NetPhos 3.1 Server、Meme和Wolf Psort工具获取烟草丙酮酸脱羧酶的磷酸化位点、保守基序和亚细胞定位的相关信息并进行分析。结果表明:丝氨酸和苏氨酸磷酸化位点数以NtPDC_1成员较多,酪氨酸磷酸化位点数以以NtPDC_3成员较多;烟草丙酮酸脱羧酶成员中共检测到7组保守基序,以Motif 1~6较为保守,NtPDC_6缺失Motif 7,除Motif 5外,其他他保守基序均作为序列功能结构域的重要组成部分;NtPDC_1、NtPDC_2和NtPDC_3定位于细胞质,NtPDC_4定位于线粒体,NtPDC_5和NtPDC_6定位于内质网。

In order to further understand the characteristics and functions of tobacco pyruvate decarboxylase,NetPhos 3.1 Server,Meme and Wolf PSORT tools were used to obtain and analyze the phosphorylation sites,conserved motifs and subcellular localization of tobacco pyruvate decarboxylase.The results showed that the number of phosphorylation sites of serine and threonine was ntpdc_1,the number of tyrosine phosphorylation sites was ntpdc_3,7 groups of conserved motifs were detected in pyruvate decarboxylase,motif 1~6 were conserved,and motif 7 was absent in ntpdc_6.Except motif 5,other conserved motifs were all important components of sequence functional domain Ntpdc,ntpdc and ntpdc were located in the cytoplasm,ntpdc,ntpdc and ntpdc were located in the mitochondria,ntpdc,ntpdc and ntpdc were located in the endoplasmic reticulum.