FGF-21、MIP-2在特发性肺纤维化中的变化及临床意义

Study of changes and clinical significance of FGF-21 and MIP-2 in idiopathic pulmonary fibrosis

目的研究成纤维细胞生长因子21(FGF-21)、巨噬细胞炎性蛋白2(MIP-2)在特发性肺纤维化中的变化及其临床意义。方法选取2013年12月-2018年12月海南省人民医院确诊的特发性肺纤维化患者80例,取患者肺纤维化组织为研究对象,取肺旁正常组织作为对照,Western blotting检测FGF-21和MIP-2蛋白的相对表达量,逆转录聚合酶链反应(RT-PCR)检测FGF-21 mRNA和MIP-2 mRNA的表达水平。比较两组FGF-21和MIP-2蛋白和mRNA的表达水平及其与临床病理特征的关系。利用条件Logistic回归分析特发性肺纤维化的相关因素,利用Cox回归分析特发性肺纤维化患者临床预后的相关因素。结果特发性肺纤维化组织FGF-21mRNA、MIP-2 mRNA及其蛋白的表达均较正常组织升高(P<0.05);FGF-21 mRNA和MIP-2 mRNA的表达与患者年龄、性别、吸烟、BMI和病程无关(P>0.05)。条件Logistic回归分析模型结果表明,特发性肺纤维化的发生与FGF-21 mRNA、MIP-2 mRNA呈正相关(P<0.05);Cox回归分析结果显示,FGF-21 mRNA、MIP-2 mRNA的高表达是影响特发性肺纤维化患者临床预后的独立危险因素。结论 FGF-21 mRNA、MIP-2 mRNA的高表达是特发性肺纤维化发生的相关因素,并与临床预后相关。

Objective To investigate the changes and clinical significance of FGF-21 and MIP-2 in idiopathic pulmonary fibrosis.Methods Eighty patients with idiopathic pulmonary fibrosis in our hospital from December 2013 to December 2018 were selected as the research objects.The pulmonary fibrosis tissue of the patients was taken as the research object,and the adjacent normal tissue was taken as the control.The levels of FGF-21 and MIP-2 were determined by Western blotting,while the levels of FGF-21 and MIP-2 mRNA were determined by RT-PCR.The relationship between pathologic feature and FGF-21 mRNA,MIP-2 mRNA between the two groups was compared.The related factors of idiopathic pulmonary fibrosis were analyzed by Logistic Regression analysis.The related factors of clinical prognosis of patients with idiopathic pulmonary fibrosis were analyzed by COX regression analysis.Results The levels of FGF-21 mRNA,MIP-2 mRNA of pulmonary fibrosis tissue were higher than those of normal tissue(P<0.05).There was no relationship between FGF-21 mRNA,MIP-2 mRNA and age,gender,smoking,BMI as well as disease courses(P>0.05).There was positive relationship between idiopathic pulmonary fibrosis and FGF-21 mRNA,MIP-2 mRNA(P<0.05)analyzed by Logistic Regression analysis.The high expression levels of FGF-21 mRNA and MIP-2 mRNA were independent factors affecting the clinical prognosis of patients with idiopathic pulmonary fibrosis analyzed by COX regression analysis.Conclusions The high expression levels of FGF-21 mRNA and MIP-2 mRNA are related factors in the occurrence of idiopathic pulmonary fibrosis,and are associated with clinical prognosis.