硅酸盐基-Cu/Mg生物活性陶瓷促进成骨细胞的成骨性能

Silicate-Cu/Mg bioactive ceramics promote the osteogenesis of osteoblasts

背景:硅酸盐生物陶瓷作为组织工程骨支架时促成骨能力不足。研究发现铜、镁等人体所必需微量元素对成骨细胞和成血管细胞具有明显的诱导、刺激作用。目的:探讨掺杂铜、镁元素的多元硅酸盐生物活性陶瓷对成骨细胞增殖和成骨性能的影响。方法:将铜、镁加入硅酸盐生物活性陶瓷中,采用溶胶-凝胶法制备掺铜硅酸盐生物陶瓷、掺镁硅酸盐生物陶瓷与掺铜镁硅酸盐生物活性陶瓷(陶瓷中铜、镁的摩尔百分比均为5%),分别记为CS-5Cu、CS-5Mg、CS-5Cu/5Mg,以硅酸盐生物活性陶瓷为对照(记为CS),利用X射线衍射、傅里叶红外光谱对样本进行表征,检测生物陶瓷表面析晶情况。将成骨细胞分别与4组陶瓷共培养24 h,分析成骨细胞的增殖指数、碱性磷酸酶分泌量、骨桥蛋白和骨钙素基因表达及黏着斑和肌动蛋白表达。结果与结论:①陶瓷矿化结晶能力大小顺序为:CS-5Cu>CS>CS-5Cu/5Mg>CS-5Mg;②成骨细胞增殖指数的大小顺序为:CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS;③碱性磷酸酶分泌量的大小顺序为:CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS;④骨桥蛋白和骨钙素基因表达的高低顺序为:CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS;⑤黏着斑和肌动蛋白表达多少的顺序为:CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS;⑥结果表明,掺铜或掺镁硅酸盐生物活性陶瓷可促进成骨细胞的增殖、成骨相关基因表达及其黏附与铺展,且同时掺入两种离子硅酸盐生物活性陶瓷的促进效果最明显。

BACKGROUND: The use of silicate bioceramics as a tissue-engineered bone scaffold has poor ability to promote osteogenesis. Studies have shown that copper, magnesium, and other essential trace elements have obvious effects on the induction and stimulation of osteoblasts and hemangioblasts. OBJECTIVE: To investigate the effects of silicate bioactive ceramics with Cu and Mg on osteoblast proliferation and osteogenesis. METHODS: Cu-silicate bioceramics, Mg-silicate bioceramics, and Cu-Mg-silicate bioactive ceramics were prepared by the sol-gel method(molar ratio of both Cu and Mg in ceramics was 5%). Three experimental groups were CS-5Cu, CS-5Mg, CS-5Cu/5Mg groups. The silicate bioactive ceramics served as the control group(denoted as CS). X-ray diffraction(XRD) and Fourier transform infrared(FTIR) spectroscopy were used to characterize the samples. The surface crystallization of bioceramics was detected. Osteoblasts were co-cultured with four groups of ceramics for 24 hours. Osteoblast proliferation index, alkaline phosphatase secretion, osteopontin and osteocalcin gene expression, vinculin and actin protein expression were determined. RESULTS AND CONCLUSION:(1) The crystallization ability of different silicate bioceramic samples followed the order of CS-5Cu>CS>CS-5Cu/5Mg>CS-5Mg.(2) Osteoblast proliferation index followed the rule of CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS.(3) Alkaline phosphatase secretion was in the order of CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS.(4) Osteopontin and osteocalcin gene expression followed the rule of CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS.(5) Vinculin and actin protein expression was in the order of CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS.(6) These results suggest that Cu-or Mg-silicate, in particular Cu-Mg-silicate bioactive ceramics can promote the proliferation of osteoblasts and the expression of osteogenesis-related genes as well as cell adhesion and spreading.