基于Oncomine数据库及生物信息学方法挖掘卵巢癌PSME2基因表达意义及作用机制

Excavating significance and action mechanism of ovarian cancer PSME2 gene expression based on Oncomine database and bioinformatics methods

目的基于Oncomine数据库及cBioPortal挖掘人蛋白酶激活复合体亚基2(PSME2)基因在卵巢癌细胞中的表达及可能的作用机制,为深入研究PSME2在卵巢癌中的作用提供理论依据。方法对Oncomine数据库中PSME2基因在卵巢癌中表达的13项研究数据进行荟萃分析,在cBioPortal在线数据库应用Kaplan-Meier法分析PSME2表达水平与卵巢癌患者生存时间的关系,探讨其临床意义,应用Genecards数据库收集与PSME2基因相关的蛋白,并通过STRING绘制PSME2相关蛋白网络图,随后结合DAVID在线软件分析蛋白富集的生理过程。结果挖掘Oncomine数据库中关于卵巢癌细胞及正常卵巢细胞中PSME2基因表达的13项研究数据,卵巢癌中PSME2基因的表达显著高于正常卵巢(P<0.05)。Kaplan-Meier生存曲线分析发现,卵巢癌患者中高表达PSME2基因的无病生存率、总生存率均显著低于低表达组(P<0.05)。通过Genecards数据库收集到与PSME2相关的蛋白有TRAF2、RELB、NFKBIA、VHL等25个,其相关蛋白富集分析结果显示PSME2相关蛋白主要富集在蛋白酶体介导的泛素依赖型蛋白分解代谢、免疫监视及免疫杀伤、病毒致癌、调控细胞转录、细胞分化、细胞周期等生理过程。结论PSME2基因在卵巢癌组织中呈高表达,其可能通过调节蛋白酶体介导的蛋白分解代谢,使肿瘤细胞逃脱免疫监视与免疫杀伤而发挥癌基因作用,该基因可能作为卵巢癌诊治的一个靶点,但其具体的作用机制需要进一步研究和实验验证。

Objective To excavate the significance and possible action mechanism of human proteasome activator complex subunit 2(PSME2)gene expression in ovarian cancer cells based on the Oncomine database and cBioPortal to provide a theoretical basis for the in-depth study of PSME2 role in ovarian cancer.Methods The 13-item research data of PSME2 gene expression in Oncomine database conducted the meta analysis.The relationship between PSME2 expression level and survival time of the patients with ovarian cancer was analyzed by using the kaplan-meier method in the cBioPortal on-line database,and its clinical significance was discussed.The proteins related to PSME2 gene were collected by using the Genecards database,and the PSME2-related protein network chart was drawn by STRING,and then the physiological process of protein enrichment was analyzed by DAVID on-line software.Results The 13-item study data of PSME2 gene expression in ovarian cancer cells and normal ovarian cells were excavated from Oncomine database,and the PSME2 gene expression in ovarian cancer was significantly higher than that in normal ovary(P<0.05).The Kaplan-meier survival curve analysis found that the disease-free survival rate and overall survival rate in the ovarian cancer patients with high expression of PSME2 gene were significantly lower than those in the low-expression group(P<0.05).Through the Genecards database,25 proteins related to PSME2 were collected,such as TRAF2,RELB,NFKBIA,VHL.Their related protein enrichment analysis results showed that the PSME2 related proteins mainly enriched in the proteasome mediated ubiquitin dependent protein catabolism,immune surveillance and immune killing,viruses caused cancer,regulating the physiological processes of cell transcription,cell differentiation,cell cycle,etc.Conclusion PSME2 gene is highly expressed in ovarian cancer tissues,which may play an oncogene role by regulating proteasome-mediated protein catabolism,enabling tumor cells to escape immune surveillance and immune killing.This gene may be a target in the diagnosis and treatment of ovarian cancer,but its specific mechanism needs to be further studied and verified.