纳米复合材料GO@AgPt对非小细胞肺癌的放射增敏作用

Radiosensitizing effect of GO@AgPt nanocomposite on non-small-cell lung cancer

目的:制备新型纳米复合材料GO@AgPt,探究其对非小细胞肺癌的放射增敏效果。方法:通过改进型的水热法合成纳米复合材料GO@AgPt,通过透射电子显微镜、原子力显微镜、X射线光电子能谱分析、傅里叶红外光谱仪等方法对材料进行形貌及组分分析。应用细胞活性检测试剂盒CCK-8检测材料对肺癌细胞A549和人肺微血管细胞HPMEC的细胞活性的影响;然后通过流式检测及细胞克隆形成实验探究材料对于A549细胞的放射增敏效果。结果:成功制备出粒径大小均匀、化学成分稳定的纳米复合材料GO@AgPt。在一定浓度范围内该材料对肺癌细胞A549有毒性而对正常细胞HPMEC没有明显毒性。该材料能促进癌细胞产生活性氧,与X射线联合作用能促进细胞的凋亡。细胞克隆形成的结果也显示该材料对肺癌细胞具有放射增敏效果。结论:成功制备了纳米复合材料GO@AgPt,证明了该材料对于非小细胞肺癌有明显的放疗增敏效果。

Objective To prepare a novel GO@AgPt nanocomposite as a new radiotherapy sensitizer and investigate its radiosensitizing effects on non-small-cell lung cancer(NSCLC). Methods GO@Ag Pt nanocomposite was prepared by an improved hydrothermal method. Transmission electron microscope, atomic force microscope, X-ray photoelectron spectroscopy and Fourier transform infrared spectrometer were utilized to characterize the surface morphology of GO@AgPt nanocomposite and perform an analysis on the chemical compositions. The cytotoxicities of GO@Ag Pt nanocomposite on NSCLC cells(A549 cells) and human pulmonary microvascular endothelial cells(HPMEC cells) were evaluated by cell counting kit-8(CCK-8) assay, and the radiosensitization effects of GO@Ag Pt nanocomposite on A549 cells was determined by colony formation test and flow cytometer.Results GO@AgPt nanocomposite with uniform particle size and stable chemical compositions was successfully prepared. In a certain concentration range, GO@Ag Pt nanocomposite had a toxic effect on A549 cells, and meanwhile HPMEC cells were not affected. The nanocomposite enhanced the reactive oxygen production in A549 cells, and the combination with X-ray promoted cell apoptosis. Furthermore, the colony formation test showed that GO@Ag Pt nanocomposite had radiosensitizing effects on A549 cells. Conclusion GO@Ag Pt nanocomposite is successfully prepared and is proved to be a promising radiotherapy sensitizer for NSCLC.