脓毒症患者血清lncRNA IFNG-AS1的表达及临床意义

Expression and clinical significance of serum lncRNA IFNG-AS1 in sepsis patients

目的检测脓毒症患者血清中lncRNA IFNG-AS1的表达,并探究其临床意义。方法qRTPCR检测血清IFNG-AS1水平;ROC曲线分析其对脓毒症的诊断价值及区分不同预后的价值;相关性分析研究IFNG-AS1与APACHE II评分、CRP及炎性细胞因子的关系。结果脓毒症患者血清中IFNG-AS1高表达,并可作为诊断脓毒症的标志物;IFNG-AS1的表达与APACHEⅡ评分、CRP及TNF-α、IL-1β、IL-6、IL-8呈正相关,而与IL-10呈负相关;死亡患者的IFNG-AS1表达显著高于生存患者,其表达可作为评估脓毒症患者预后的标志物。结论脓毒症患者血清IFNG-AS1表达升高,可作为潜在的生物学标志物用于疾病诊断、提示疾病严重程度和机体炎症状态以及评估预后。

Objective To detect the expression of IncRNA IFNG-AS1 in serum of sepsis patients and explore its clinical significance.Methods Serum IFNG-AS1 level was detected by qRT-PCR;ROC curve was used to analyze the value of IFNG-AS1 in diagnostic sepsis and to distinguish different prognosis.Correlation analysis was used to study the relationship between IFNG-AS1 and APACHEⅡscore,CRP and inflammatory cytokines.Results The expression of IFNG-AS1 in serum of sepsis patients was increased and could be used as a marker for diagnosis of sepsis.The expression of IFNG-AS1 was positively correlated with APACHEⅡscore,CRP and TNFalpha,IL-1β,IL-6 and IL-8 levels,but negatively correlated with IL-10 level.The expression of IFNG-AS1 in dead patients was significantly higher than that in survivors,and its expression could be used as a marker to evaluate prognosis of patients with sepsis.Conclusion The increased expression of serum IFNG-AS1 in sepsis patients could be used as a potential biological marker for disease diagnosis,indicating the severity of disease and inflammation status,and evaluating prognosis.