大白菜BrROP基因家族的生物信息学分析

Bioinformatics Analysis of BrROP Gene Family in Chinese Cabbage

为了揭示大白菜BrROP基因家族的功能和进化关系,利用生物信息学方法对大白菜BrROP基因家族成员进行了鉴定,并对其基因结构、蛋白质序列、染色体定位、保守结构域、进化关系和表达模式等进行系统分析。结果表明,在大白菜基因组中共鉴定出22个BrROP基因成员,在大白菜染色体上呈不均匀分布;氨基酸序列分析表明,BrROP蛋白的结构较保守,均含有G结构域(G1—G5)、效应因子结合点、插入序列和C端可变区;根据系统发育进行聚类分析,大白菜BrROP基因可被分为4类,GroupⅠ、GroupⅡ、GroupⅢ和GroupⅣ;利用EMBL-EBI数据库对BrROP基因家族进行分析,22个BrROP基因在大白菜根、茎、叶、花和角果中呈组织差异表达。

To reveal the function and phylogenetic relationship of the BrROP gene family in Chinese cabbage,bioinformatics methods were used to identify the BrROP gene family members of Chinese cabbage,and the gene structure,protein sequence,chromosomal distribution,conserved domain,phylogenetic relationship and expression pattern were systematically analyzed.The results showed that 22 BrROP genes were identified in the Chinese cabbage genome,which were unevenly distributed on the chromosome of Chinese cabbage.Amino acid sequence analysis indicated that the structure of BrROP protein was conserved,including G domain(G1-G5),effector binding site,Rho insert region and C-terminal hypervariable region.According to the phylogenetic analysis,BrROP genes were divided into four categories,GroupⅠ,GroupⅡ,GroupⅢand GroupⅣ.The expression of BrROP genes was analyzed using the EMBL-EBI database.22 BrROP genes were differentially expressed in root,stem,leaf,flower and silique.