姜黄素的提纯与分析

Purification and Analysis of Curcumin

采用溶剂萃取法从姜黄素类混合物中提纯姜黄素.对萃取剂种类、萃取剂浓度、萃取时间等萃取条件进行优化,确定出最佳萃取条件.再用薄层色谱法分离姜黄素,薄层色谱法以三氯甲烷-甲醇-甲酸(体积比为96∶3∶1)为展开剂,用姜黄素标准品进行对照,姜黄素的Rf值为0.71,与其他组分得到分离.采用液相色谱法和红外光谱法进行分析鉴定姜黄素,结果满意.

The solvent extraction method was used to purify curcumin from curcumin mixture.The extraction conditions,such as extraction type,extraction concentration and extraction time,were optimized to determine the best extraction conditions.Then the curcumin was separated by thin layer chromatography(TLC).Chloroform∶methanol∶formic acid=96∶3∶1 was used as the development agent and curcumin standard was used as the control.The Rf value of curcumin was 0.71,and it was separated from other components.Curcumin was identified by liquid chromatography and infrared spectroscopy with satisfactory results.