养殖场除臭酵母发酵条件的优化

Optimization of Fermentation Conditions for Deodorant Saccharomycetes in Livestock Farm

为了规模化生产除臭酵母菌JM-1,以菌体活菌数为指标,通过单因素试验和正交试验对其发酵条件进行了优化。结果表明:酵母菌JM-1的最佳发酵条件为:培养温度30℃,初始pH值 5.0,接种量4%,500 mL三角瓶装液量150 mL。在此条件下培养24 h,酿酒酵母的发酵菌数超过7.42×10^8 CFU/mL,与优化前的6.11×10^8 CFU/mL相比较,活菌数提高了21.1%。

In order to obtain the optimal fermentation condtions of a strain of JM-1,the fermentation conditions were optimized by single factor experiment and orthogonal experiment,using the number of viable bacteria in the fermentation as an index.The optimal fermentation conditions were:culture temperature 30℃,initial pH value 5.0,inoculum size 4%,and liquid filling quantity 150 mL in the 500 mL conical flask.In these conditions,the number of viable bacteria exceed 7.42×10^8 CFU/mL,which was increased by 21.1%compared to that before optimization.