摘要
为探究胎牛血清(FBS)在17℃负压环境下对猪精液的保存效果,延长猪精液保存的时间以及维持猪精子的质量。通过在Modena基础稀释液中分别添加体积分数为0、5%灭活、10%灭活、非灭活5%、非灭活10%的FBS,检测猪精子在此环境下5 d内的精子活率、直线速度、侧摆幅度等为检测参数。结果表明:10%灭活的FBS在猪精子各项参数上都显著优于其他组(P<0.05)。由结果可知,在Modena基础稀释液添加10%灭活的FBS在17℃负压环境下最有利于猪精液的保存,为猪精子的常温保存研究提供实验依据。
To explore the effect of fetal bovine serum(FBS)on the preservation of pig semen under 17℃negative pressure environment,prolong the preservation time of pig semen and maintain the quality of pig sperm,adding the volume fraction in Modena foundation diluent respectively 0,5%inactivated,10%inactivated,5%lived,and 10%lived FBS,detect the pig sperm in the environment within the 5 d’s live sperm rate,linear velocity,side swing range,etc.The results showed that 10%inactivated FBS was significantly better than other groups in various parameters of pig sperm(P<0.05).The result showed that in Modena diluent adding 10%inactivated FBS in 17℃under negative pressure environment most conducive to the pig semen preservation,which would provide experiment basis for the research on the pig sperm normal temperature preservation.
作者
李井春
王硕
李雁冰
王国鑫
魏国生
Li Jingchun;Wang Shuo;Li Yanbing;Wang Gouxin;Wei Guosheng(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319)
出处
《黑龙江八一农垦大学学报》
2020年第2期27-31,67,共6页
journal of heilongjiang bayi agricultural university
基金
黑龙江省自然科学基金面上项目(C2017053)
黑龙江省农垦总局科研计划项目(HNK135-04-06)
黑龙江八一农垦大学青年创新人才项目(CXRC2017004)
黑龙江八一农垦大学博士启动基金(XYB-2016-04)
黑龙江省博士后科研启动基金(LBH-Q18100)。
关键词
胎牛血清
猪精子
17℃
负压
fetal bovine serum(FBS)
pig sperm
17℃
negative pressure