高精度时空转录组揭示小鼠早期胚胎三胚层细胞谱系发生过程

Lineage Specification of Three Germ Layers of Early Mouse Embryo by Spatiotemporal Transcriptome

从受精卵发育成具有不同细胞类型个体的过程中,细胞命运受到多个层次的调控。在哺乳动物的早期胚胎发育过程中,原肠运动是外、中、内三个胚层的建立过程,为后续的器官发生和形态建成提供了发育蓝图。然而目前对于三胚层命运建立的分子机制认识并不清晰。该文通过对小鼠早期胚胎的时空转录组分析,从分子层面揭示了外、中、内三胚层谱系发生的整个过程。

Cell fate specification in the development of zygote into embryo proper is regulated at multiple levels.During the early embryonic development of mammals,formation of the three primary germ layers,ectoderm,mesoderm,and endoderm,as the results of gastrulation,provides a blueprint for morphogenesis and organogenesis.However,a comprehensive genome-wide molecular annotation of the mechanisms that determine the tissue architecture and lineage specification of the three germ layers has not been clarified.We show that the entire process of the specification of the ectoderm,mesoderm and endoderm lineages is revealed by analyzing the spatiotemporal transcriptomes of mouse early embryos.