摘要
有别于传统的通过富集分离纯化技术获得一些纯培养污染物降解功能微生物,稳定同位素技术(SIP-DNA)可以更加便捷地鉴定出功能基因。该文研究针对SIP-DNA中密度梯度离心这一核心技术,获得了该技术的条件及相关参数。浮力密度和DNA浓度结果表明该方法可以有效地把同位素标记和非同位素标记的DNA分离,为SIP-DNA后续高通量测序奠定基础。同时也为离心机管理人员和实验人员正确使用该方法提供技术手段。
Unlike the traditional enrichment,separation and purification technology to obtain some purely cultured pollutant-degrading functional microorganisms,stable isotope technology(SIP-DNA)can more easily identify functional genes.In this study,the core technology of density gradient centrifugation in SIP-DNA was used to obtain the conditions and related parameters of the technology.The buoyancy density and DNA concentration results show that this method can effectively isolate isotope-labeled and non-isotopic-labeled DNA and lay the foundation for subsequent high-throughput sequencing of SIP-DNA.It also provides technical means for centrifuge managers and experimenters to use the method correctly.
作者
何秋香
HE Qiuxiang(College of Resources and Environment,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《实验科学与技术》
2020年第2期32-35,共4页
Experiment Science and Technology
关键词
稳定同位素
密度梯度离心
功能微生物
浮力密度
stable isotope technique
density gradient centrifugation
functional microorganism
buoyant density
