HPLC-DAD双波长法同时测定小秦艽花中5个成分的含量

Simultaneous determination of five components in flowers of Gentiana dahurica by HPLC-DAD dual wavelength method

目的:建立HPLC法测定蒙药小秦艽花中大麦黄苷、马钱苷、肥皂草苷、异荭草苷和异牡荆苷的含量。方法:采用Symmetry?C18色谱柱(4.6 mm×250 mm,5.0μm),以乙腈(A)-0.1%甲酸水溶液(B)为流动相,梯度洗脱,流速为1.0 mL·min-1,柱温35℃,检测波长为245 nm和330 nm。结果:大麦黄苷、马钱苷、肥皂草苷、异荭草苷和异牡荆苷5个成分的进样量分别在0.124~2.484μg (r=0.999 3)、0.127~2.540μg (r=1.000)、0.015~0.302μg (r=0.999 9)、0.112~2.396μg (r=1.000)、0.024~0.482μg (r=0.999 7)范围内线性关系良好;平均回收率(n=3)在96.5%~102.8%范围内,RSD为0.88%~2.8%。16批小秦艽花样品中大麦黄苷、马钱苷、肥皂草苷、异荭草苷和异牡荆苷的含量范围分别为1.15~7.71、0.38~9.23、0.13~0.86、1.11~7.46、0.59~1.70 mg·g-1。结论:该方法可以用于小秦艽花中5个活性成分的含量测定,为小秦艽花药材的资源开发提供科学依据。

Objective:To establish an HPLC method for simultaneous determination of lutonarin,loganin,saponarin,isoorientin and isovitexin in flowers of Gentiana dahurica. Methods:The analysis was performed on Symmetry? C18 column(4.6 mm×250 mm,5.0 μm) with acetonitrile(A)-0.1% formic acid(B) as mobile phase with gradient elution. The flow rate was 1.0 mL·min-1,the column temperature was 35 ℃,and the detection wavelengths were 245 nm and 330 nm. Results:There were good linearity in the ranges of 0.124-2.484 μg(r=0.999 3) for lutonarin,0.127-2.540 μg(r=1.000) for loganin,0.015-0.302 μg(r=0.999 9) for saponarin,0.112-2.396 μg(r=1.000) for isoorientin and 0.024-0.482 μg(r=0.999 7) for isovitexin. The average recoveries(n=3) of five components were in the range of 96.5%-102.8%,and the RSDs were in the range of 0.88%-2.8%. The content ranges of lutonarin,loganin,saponarin,isoorientin,isovitexin in the 16 batches of flowers of G. dahurica were 1.15-7.71 mg·g-1,0.38-9.23 mg·g-1,0.13-0.86 mg·g-1,1.11-7.46 mg·g-1 and 0.59-1.70 mg·g-1,respectively. Conclusion:The method can be used for the determination of five index chemical components in flowers of G. dahurica,providing a scientific basis for the resource development of flowers of G. dahurica.