多烯紫杉醇石墨烯的制备及质量评价

Preparation and quality evaluation of functional graphene oxied/docetaxel

目的:合成新型的多烯紫杉醇石墨烯复合材料(Fe3O4/GO-NA/DOC),并对其进行质量评价。方法:利用透射电镜观察其形貌;以高效液相色谱法(HPLC)测定多烯紫杉醇(DOC)的含量,并对生成物的包封率、回收率以及精密度进行评价,以低温高速离心法测定Fe3O4/GO-NA/DOC复合材料的包封率。结果:多烯紫杉醇检测浓度线性范围为20~120μg·mL-1(r=0.9991),平均回收率(n=3)为100.2%±0.23%,在转速为20000 r·min-1,离心时间为80 min的条件下,包封率的值在90%以上。结论:Fe3O4/GO-NA/DOC复合材料制备工艺可行,质量控制方法简便可靠。

Objective:To synthetize the new-type Fe3 O4/GO-NA/DOC composite materials,and evaluate their qualities.Methods:The morphology was observed by transmission electron microscope.The concentration of DOC was determined by high efficiency liquid chromatography.The recovery,precision and entrapment efficiency of Fe3 O4/GO-NA/DOC composite materials were studied,and the Fe3 O4/GO-NA/DOC composite materials entrapment efficiency was determined by Low temperature high speed centrifugation.Results:The linear detection concentration range of DOC was 20-120μg·mL-1(r=0.9991)with the average recovery(n=3)of 100.2%±0.23%.At a speed of 20000 r·min-1 and a centrifugation time of 80 min,the encapsulation rate was more than 90%.Conclusion:The preparation technique of Fe3 O4/GO-NA/DOC composite materials is feasible,and the quality control method is convenient and reliable.