摘要
为选育出适合北方海域环境、具有优良经济性状的菲律宾蛤仔Ruditapes philippinarum良种,以菲律宾蛤仔(以下简称蛤仔)中国莱州群体(C)与朝鲜新义州(K)群体为亲本,采用双列杂交方法建立2个自繁群体CC(C♀×C♂)和KK(K♀×K♂)、1个正交群体CK(C♀×K♂)和1个反交群体KC(K♀×C♂)。结果表明:CC组受精率最高,为(96.23±1.67)%,CK组受精率最低,为(88.80±1.84)%;CC组孵化率最高,为(86.80±0.78)%,KC组孵化率最低,为(72.05±0.82)%;在幼虫期,生长和存活总体表现为杂种劣势,壳长和壳高在15日龄时由大至小依次为CC>CK>KK>KC,15日龄时CK组存活率为(33.00±3.51)%,KC组存活率为(13.40±4.45)%;在稚贝期,生长和存活表现出杂种优势,壳长90日龄和壳高120日龄生长的杂交优势分别达到最大,分别为16.91%和12.53%,120日龄时KC组存活率最高,为(79.82±1.02)%,KK组存活率最低,为(65.51±1.58)%;在45、90和120日龄,C群体父母本的壳长、壳高都具有正向的一般配合力(GCA),说明中国莱州群体可作为优良亲本与其他群体组配;KC组的特殊配合力(SCA)在90和120日龄后逐渐大于CK组。研究表明,以中国莱州群体为父本与朝鲜新义州群体为母本杂交效果较好。
China Laizhou(C)and north Korea Sinǔiju(K)populations of wild Manila clam Ruditapes philippinarum as the parents were diallelly crossed to produce 2 self propagating populations of CC(C♀×C♂)and KK(K♀×K♂),and 2 reciprocal cross groups of CK(C♀×K♂)and KC(K♀×C♂)in order to culture fine species of Manila clam with good economic characters and suitable for northern sea environment.The results showed that there was the maximal fertilization rate(96.23±1.67)%in group CC,and the minimal one(88.80±1.84)%in group CK.The maximal hatching rate(86.80±0.78)%was observed in group CC,and the minimal one(72.05±0.82)%in KC group.In the larval stage,the heterosis of growth and survival was observed in the hybrid,the descending order of the shell length and shell height at 15 days old was expressed as CC>CK>KK>KC,with survival rate of(33.00±3.51)%in group CK and(13.40±4.45)%in group KC.In the juvenile period,the 90 and 120 days old hybrids had heterosis of growth and survival,with the maximal shell length(16.91%)in 90 days old hybrids and the maximal shell height(12.53%)in the 120 days old hybrid,the maximal survival rate in 120 days old hybrid in group KC(79.82±1.02)%,and the minimal survival rate in group KK(65.51±1.58)%.In 45,90 and 120 days old juveniles,parents in group C had positive general combining ability(GCA)in shell length and shell height,indicating that China Laizhou groups can be used as a good parent equipping with other groups.There was greater special combining ability(SCA)in 90 and 120 days old juveniles in group KC than hat in group CK.The findings indicated that the Chinese Laizhou population as paternal parent and the Korean Sinǔiju population as the maternal parent had a better hybridization effect.
作者
李金龙
刘越
车宗豪
田园
刘杰
闫喜武
霍忠明
LI Jinlong;LIU Yue;CHE Zonghao;TIAN Yuan;LIU Jie;YAN Xiwu;HUO Zhongming(College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province,Dalian 116023,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2020年第2期190-196,共7页
Journal of Dalian Ocean University
基金
国家重点研发计划项目“蓝色粮仓科技创新项目重要养殖贝类种质创制与规模化制种”(2018YFD0901400)
国家贝类产业技术体系专项(CARS-49)
辽宁省“兴辽英才计划项目”(XLYC1807271)
辽宁省高等学校海洋产业技术研究院项目(2018-CY-31)
大连市支持高层次人才创新创业项目(2017RQ062)。
关键词
菲律宾蛤仔
双列杂交
配合力
杂种优势
Ruditapes philippinarum
diallel cross
combining ability
heterosis