七氟烷对心肌缺血再灌注内皮细胞细胞间黏附分子-1、血管细胞黏附分子-1和E-选择素表达的影响

Effect of sevoflurane on the expression of ICAM-1,VCAM-1 and E-selectin in endothelial cells after myocardial ischemia-reperfusion

目的:探讨七氟烷对心肌缺血再灌注内皮细胞促炎作用的细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)和E-选择素表达的影响。方法:在大鼠心肌缺血再灌注模型的基础上,将大鼠随机分为假手术组(对照组)、缺血再灌注损伤组(I/R组)和七氟烷组;观察各组大鼠手术前、缺血15 min和再灌注4 h的心率、平均动脉压和心率-收缩压乘积(RPP);免疫组织化学法检测心肌组织中CD68^+巨噬细胞数目、内皮细胞ICAM-1、VCAM-1和E-选择素的表达;TUNEL染色法检测凋亡细胞的比例。结果:缺血15 min时,I/R组和七氟烷组平均动脉压和RPP均显著下降;再灌注4 h时,七氟烷组平均动脉压和RPP均有所上升,相对于I/R组,差异具有统计学意义;与对照组比较,I/R组内皮细胞ICAM-1、VCAM-1和E-选择素的表达均显著升高,七氟烷则能够有效抑制I/R引起的内皮细胞促炎分子的表达;对照组CD68^+巨噬细胞为5.83个/高倍镜视野(HPF),I/R组数目为55.67个/HPF,两组间差异具有统计学意义;七氟烷能够显著减少心组织内巨噬细胞的浸润,与I/R组比较,降低了66.46%;TUNEL染色结果显示对照组心肌细胞凋亡率2.20%,I/R组为28.63%,两组间差异明显;七氟烷能够显著降低心肌细胞的凋亡,相对于I/R组,降低了51.76%。结论:七氟烷可降低缺血再灌注损伤后内皮细胞表面促炎分子的表达,减少心肌组织巨噬细胞浸润和细胞凋亡。

Objective:To investigate the effects of sevoflurane on the expression of ICAM-1,VCAM-1 and E-selectin in endothelial cells induced by myocardial ischemia-reperfusion.Methods:Thirty rats were randomly divided into three groups:sham operation group(control group),ischemia reperfusion injury group(I/R group)and sevoflurane group.The heart rate,mean arterial pressure and heart rate systolic pressure product(RPP)of rats in each group were observed before operation,15 minutes after ischemia and 4 hours after reperfusion,respectively.The number of CD68^+macrophages,and the expression of ICAM-1,VCAM-1 and E-selectin in endothelial cells were detected by immunohistochemistry.The proportion of apoptotic cells was detected by TUNEL staining.Results:The mean arterial pressure and RPP decreased significantly in I/R group and sevoflurane group at 15 min of ischemia reperfusion,and increased at 4 h of ischemia compared with I/R group.Compared with the control group,the expression of ICAM-1,VCAM-1 and E-selectin in endothelial cells of I/R group increased significantly,while sevoflurane could effectively inhibit the expression of inflammatory molecules in endothelial cells induced by I/R.CD68^+macrophages in control group were 5.83/high power view(HPF)and 55.67/HPF in I/R group,and there was significant difference between the two groups.Sevoflurane reduced the infiltration of macrophages,which was 66.46%lower than that of I/R group.TUNEL staining showed that the apoptosis rate of cardiomyocytes was 2.20%in control group and 28.63%in I/R group.The difference between the two groups was significant.Sevoflurane could reduce the percentage of apoptotic cells,which was 51.76%lower than that in I/R group.Conclusion:Sevoflurane can reduce the expression of pro-inflammatory molecules on endothelial cells after ischemia-reperfusion injury,and reduce the infiltration of macrophages and cell apoptosis in myocardial tissue.