石榴WRKY基因家族全基因组鉴定与表达分析

Genome-wide Identification and Expression Analysis of WRKY Gene Family in Punica granatum

WRKY蛋白是一类在植物生长发育过程及生物与非生物胁迫过程中起重要调控作用的转录因子。该研究利用石榴全基因组数据,采用生物信息学的方法,对石榴WRKY转录因子家族成员蛋白理化性质、系统进化、基因结构、保守基序、顺式作用元件、蛋白互作及基因共表达和转录组表达模式进行系统分析。结果共鉴定出69个PgWRKY基因;分组鉴定和进化分析显示WRKY蛋白可分为Ⅰ、Ⅱ和Ⅲ共三大类型。顺式作用元件分析表明,PgWRKY基因广泛参与到非生物胁迫中;蛋白互作网络与共表达分析暗示PgWRKY基因在同一胁迫应答中可能作用一致并同时诱导表达;RNA-Seq数据分析表明,PgWRKY基因有一定的组织表达特异性,广泛参与植物营养、生殖生长以及根部逆境胁迫应答过程。

As a family of transcription factors,WRKY protein plays an important regulatory role in plant growth and development and in response to environmental signals.In this study,the whole genome data of pomegranate were used to systematically analyze the proteins'physicochemical properties,phylogenetic evolution,gene structure,conserved motifs,cis-acting elements,protein interaction,gene co-expression,and transcriptome expression patterns of pomegranate WRKY transcription factors by bioinformatics.We identified 69 genes.PgWRKY gene group identification and evolutionary analysis show that WRKY proteins can be divided intoⅠ,ⅡandⅢaltogether three types.Cis-acting element analysis showed that PgWRKY gene was widely involved in abiotic stress.Protein interaction network and co-expression analysis suggested that PgWRKY gene might play a consistent role in the same stress response and induce expression simultaneously.RNA-seq data analysis indicated that PgWRKY gene had certain tissue expression specificity and was widely involved in plant nutrition,reproductive growth and root stress response.