虎杖提取物对肺炎支原体感染后小鼠心肌损伤的干预作用

Intervention effect of Polygonum cuspidatum extract on myocardial injury in mice with Mycoplasma pneumoniae infection

目的研究虎杖提取物对肺炎支原体(MP)感染后小鼠心肌损伤的干预作用。方法30只BALB/c小鼠随机分为正常组、MP模型组、虎杖提取物组,每组10只。正常组用生理盐水滴鼻,MP模型组和虎杖提取物组均用支原体菌液滴鼻感染,连续3 d。治疗从感染第1天开始,连续治疗3 d。ELISA法检测小鼠血清中肌酸激酶同工酶(CK-MB)、心肌肌钙蛋白(cTnI)、肌红蛋白(Mb)和谷草转氨酶(AST)的表达水平。结果与正常组相比,MP模型组的心肌损伤标志物水平均显著升高,差异有统计学意义(P<0.01);与MP模型组相比,虎杖提取物组显著下调了CK-MB、cTnI、Mb、AST的水平,差异有统计学意义(P<0.01)。结论虎杖提取物能降低心肌损伤标志物水平,显著改善MP感染后小鼠的心肌损伤,起到保护心脏的作用。

Objective To study the effect of Polygonum cuspidatum extract on myocardial injury in mice with Mycoplasma pneumoniae infection.Methods Totally 30 BALB/c mice were randomly divided into normal group,MP model group and Polygonum cuspidatum extract group,with 10 mice in each group.The normal group was nasally infused with normal saline,and the MP model group and Polygonum cuspidatum extract group were all nasally infected with mycoplasma bacteria solution for 3 consecutive days.Treatment started on the first day of infection and continued for 3 days.The expression level of creatine kinase isoenzyme(CK-MB),cardiac troponin(cTnI),myoglobin(Mb)and aspartate aminotransferase(AST)in serum of mice were detected by ELISA method.Results Compared with the normal group,the levels of all the myocardial injury markers in the MP model group were significantly increased(P<0.01);compared with the MP model group,in the Polygonum cuspidatum extract group CK-MB,cTnI,Mb and AST levels were significantly down-regulated(P<0.01).Conclusion Polygonum cuspidatum extract can decrease the levels of CK-MB,cTnI,Mb and AST,significantly improve myocardial damage in mice with Mycoplasma pneumoniae infection,and protect the heart.