松木层孔菌的液化与红外光谱分析

Liquefaction and infrared spectrum analysis of Phellinus pini(Thore:Fr.)Ames

【目的】优化苯酚—硫酸法液化松木层孔菌的工艺参数,分析液化前后松木层孔菌化学官能团变化,揭示其液化机理,为废弃松木层孔菌的高效利用开辟一条新途径。【方法】以松木层孔菌为原料、浓硫酸为催化剂、苯酚为液化剂,考察料液比(原料/苯酚)、液化温度、液化时间和催化剂用量对松木层孔菌液化率的影响,并通过红外光谱对松木层孔菌原料和液化残渣进行分析。【结果】4个因素对松木层孔菌液化率影响排序为料液比>液化时间>催化剂用量>液化温度,优化的液化工艺参数为:料液比1∶6、液化温度170℃、液化时间75 min、催化剂用量0.30 g,在此条件下,松木层孔菌液化率为48.64%。液化前后松木层孔菌红外光谱特征峰的形状和吸收位置均发生明显变化,3366 cm-1的活泼氢吸收峰在液化后发生红移,酰胺羰基吸收峰则分别由1697和1657 cm-1移至1701 cm-1,由1554 cm-1移至1612和1512 cm-1。【结论】采用苯酚—硫酸法可有效液化松木层孔菌,松木层孔菌含氮主要成分几丁质发生脱乙酰化反应,液化产物含有大量酚类物质,可进一步用于制备高附加值化工产品,为高等真菌菌渣的综合利用开辟新途径。

【Objective】To optimize the technical parameters of Phellinus pini(Thore:Fr.)Ames liquefaction by phenol-sulfuric acid method,analyze the changes of chemical functional groups of P.pini before and after liquefaction,reveal the liquefaction mechanism of P.pini,and open up a new way for the efficient utilization of abandoned P.pini.【Method】The effects of material-liquid ratio(raw materials/phenol),liquefaction temperature,liquefaction time and catalyst dosage on the liquefaction rate were investigated by using P.pini as raw material,concentrated sulfuric acid as catalyst and phenol as liquefaction agent.The raw materials and liquefaction residue of P.pini were analyzed by infrared spectroscopy(IR).【Result】The results showed that the four factors affecting the liquefaction rate of P.pini were in the order of raw materialsliquid ratio>liquefaction time>catalyst dosage>liquefaction temperature,and the optimum liquefaction parameters were as follows:raw materials-liquid ratio of 1∶6,liquefaction temperature of 170℃,liquefaction time of 75 min,and catalyst dosage of 0.30 g.Under this condition,the liquefaction yielding of P.pini was 48.64%.The shape and absorption position of the characteristic peaks in the infrared spectrum of P.pini changed greatly before and after liquefaction.The active hydrogen absorption peak at wave number 3366 cm-1 shifted red after liquefaction,while the amido carbonyl absorption peaks shifted from 1697 cm-1 and 1657 cm-1 to 1701 cm-1 and from 1554 cm-1 to 1612 cm-1 and 1512 cm-1.【Conclusion】The phenol-sulfuric acid method can effectively liquefy P.pini,and chitin,the main nitrogen-containing component of P.pini,was deacetylated.Liquefaction products contain a large amount of phenolic substances,can be further used to prepare high value-added chemical products,and open up a new way for the comprehensive utilization of higher fungal residue.