TPM4在胃癌细胞中的表达及其对胃癌细胞侵袭与迁移能力的影响

Expression of TPM4 in gastric cancer cells and its effect on invasion and migration of gastric cancer cells

目的观察原肌球蛋白4(tropomyosin 4,TPM4)在胃癌细胞中表达及其对胃癌细胞的侵袭与迁移能力的影响.方法用Transwell小室分离法培养高迁移性胃癌细胞与非迁移性胃癌细胞亚系,分别命名为SGC7901-invasion细胞、SGC7901-noninvasion细胞,提取两株细胞的RNA并测序.qRT-PCR及Western blot分别检测两株细胞中TPM4的表达水平.另外转染TPM4-siRNA于SGC7901-invasion细胞,干扰TPM4表达,用Transwell实验及细胞划痕实验检测TPM4对SGC7901-invasion细胞侵袭与迁移能力的影响.结果RNA测序结果显示,TPM4在SGC7901-invasion细胞中表达量是SGC7901-noninvasion细胞的3.08倍(P<0.05).qRT-PCR和Western blot结果显示,与SGC7901-noninvasion细胞相比,在SGC7901-invasion细胞中TPM4的表达显著增高(P<0.05).转染TPM4-siRNA可明显抑制SGC7901-invasion细胞中TPM4的表达.Transwell实验结果显示,干扰TPM4表达可抑制SGC7901-invasion细胞的侵袭(P<0.05).细胞划痕实验结果显示,干扰TPM4的表达可抑制SGC7901-invasion细胞的迁移(P<0.05).结论TPM4在SGC7901-invasion细胞中高表达.TPM4可促进GC7901-invasion细胞侵袭与迁移.

Objective To observe the expression of tropomyosin 4(TPM4)in gastric cancer cells and its effect on the invasion and migration abilities of gastric cancer cells.Methods Transwell chamber isolation method was used to culture high-migration gastric cancer cells and non-migration gastric cancer cell lines,named SGC7901-invasion cells and SGC7901-noninvasion cells,respectively,and then RNA was extracted from the two kinds of cells and sequenced.Western blot and qRT-PCR were used to detect the expression of TPM4 in the two kinds of cells.After TPM4-siRNA was transfected into SGC7901-invasion cells,the effects of TPM4 on the invasion and migration ability of SGC7901-invasion cells were detected by Transwell assay and cell scratch test.Results RNA sequencing revealed that the expression of TPM4 in SGC7901-invasion cells was 3.08 times that of SGC7901-noninvasion cells(P<0.05).The qRT-PCR and Western blot results showed that TPM4 expression was significantly increased in SGC7901-invasion cells compared to SGC7901-noninvasion cells(P<0.05).Transfection of TPM4-siRNA significantly inhibited TPM4 expression in SGC7901-invasion cells.Transwell results showed that the scilencing of TPM4 inhibited SGC7901-invasion cell invasion(P<0.05).Cell scratch test results show that the scilencing of TPM4 inhibited SGC7901-invasion cell migration(P<0.05).Conclusion TPM4 is highly expressed in SGC7901-invasion cells.TPM4 promotes SGC7901-invasion cell invasion and migration.