三尖杉提取物对脓毒症小鼠T细胞行为及免疫的影响

Effects of Cephalotaxus fortunei extract on T cell behavior and immunity of sepsis mice

目的研究三尖杉提取物对脓毒症小鼠T细胞行为及免疫的影响。方法 SPF级雄性BALB/c小鼠40只,其中10只作为正常对照组,其余30只建立脓毒症模型,建模成功后分为模型组、阳性对照组、三尖杉提取物组,阳性对照组使用血必净干预,三尖杉提取物组使用三尖杉酯碱干预,正常对照组与模型组腹腔注射等剂量的生理盐水。对四组小鼠T细胞增殖、细胞周期变化进行检测,观察小鼠病理组织变化,测定小鼠肿瘤坏死因子-α(TNF-α)水平、皮质醇含量、胸腺指数、中性粒细胞(PMN)吞噬功能、白细胞移行抑制指数。结果三尖杉提取物组小鼠T细胞CD25、CD69、CD71表达、T细胞增殖率、TNF-α、白细胞移行抑制指数(MII)低于阳性对照组,皮质醇含量、PMN吞噬率、胸腺指数高于阳性对照组(P<0.05)。三尖杉提取物组S、G2/M期T细胞低于阳性对照组(P<0.05)。结论三尖杉提取物通过调节下丘脑-垂体-肾上腺皮质轴,抑制T细胞的活化和增殖,改善机体免疫功能。

OBJECTIVE To observe the effect of Cephalotaxus fortunei extract on T cell behavior and immunity of sepsis mice. METHODS Totally 40 SPF male BALB/c mice were enrolled in the study, 10 of which were assigned as the normal control group, and 30 were used to establish sepsis model and were divided into the model group, the positive control group and the Cephalotaxus extract group. The positive control group was treated with Xuebijing, the Cephalotaxus extract group was treated with cephalotaxine, the normal control group and the model group were given intraperitoneal injection of equal dose of normal saline. The proliferation and cycles of the T cells of the four groups of mice were detected, the pathological tissues of the mice were observed, and the tumor necrosis factor-α(TNF-α) level, content of cortisol, thymus index, phagocytic function of neutrophils(PMN) and leukocyte migration index of the mice were determined. RESULTS The levels of expression of CD25, CD69 and CD71 of T cells, proliferation rate of T cells, TNF-α level and leukocyte migration inhibition index(MII) of the Cephalotaxus extract group were significantly lower than those of the positive control group;the content of cortisol, PMN phagocytosis rate and thymus index of the Cephalotaxus extract group were higher than those of the positive control group(P<0.05). The levels of T cells in S and G2/M phase were significantly lower in the Cephalotaxus extract group than in the positive control group(P<0.05). CONCLUSION Cephalotaxus fortunei extract can inhibit the activation and proliferation of T cells and improve the immune function of the body by regulating the hypothalamus pituitary adrenocortical axis.