大白菜TPS基因家族鉴定及其在高温胁迫下的表达分析

Identification of TPS Gene Family in Chinese Cabbage(Brassica campestris L.)and Its Expression under Heat Stress

为明确大白菜TPS(BrTPS)家族成员信息及对高温胁迫信号的响应,本研究利用生物信息学方法,在大白菜基因组数据库中鉴定了BrTPS基因家族成员,并对其理化性质、进化特征、蛋白结构及在高温胁迫下的表达模式进行分析。结果表明,大白菜全基因组含有15个TPS基因家族成员,分布于8条染色体上。除BrTPS14和BrTPS15外,其余成员各含有1个TPS和1个TPP结构域,并且所含motif的排列顺序也完全一致。理化性质分析发现,15个成员的氨基酸长度介于129~1459 aa之间,分子量大小在14.73~165.83 kD之间,大部分BrTPS蛋白为酸性蛋白和亲水蛋白,以无规则卷曲作为二级结构主要构成元件。进化分析表明,大白菜TPS基因家族成员可分为2类,其中ClassⅠ包含5个成员,ClassⅡ包含10个成员。本研究对高温胁迫前后不同组织和持续高温胁迫下叶片中的表达分析,发现大部分的BrTPS基因可对高温胁迫产生响应,但在表达规律上存在差异。这些研究结果为后续研究大白菜TPS基因提供一定参考。

In order to clarify the information of BrTPS gene family and its response to heat stress,we identified the terpene synthase(TPS)in Chinese cabbage and analyzed their physical and chemical properties,protein structure,evolutionary relationship and expression pattern under high temperature stress based on bioinformatics methods.The result showed that the whole genome of Chinese cabbage contained 15 TPS genes,which distributed on 8 chromosomes.Except for BrTPS14 and BrTPS15,the others contain one TPS and one TPP domain respectively,and the sequence of motifs was same.The length of amino acids ranged from 129 aa to 1459 aa,and the molecular weight ranged from 14.73 kD to 165.83 kD.Most of the BrTPS proteins were acidic amino acids and hydrophilic proteins,random coil was the main component of secondary structure.Evolutionary analysis showed that BrTPS could be divided into two categories,ClassI and ClassII.Q-PCR was used to analyze the expression of BrTPS gene in different tissues under high temperature stress before and after,and leaves under persistent high temperature stress.It was found that most of BrTPS genes could respond to high temperature stress,but there were differences in expression patterns.These results provide a theoretical basis for further analysis of TPS gene function in Chinese cabbage.