欧李ChCBF1基因的克隆及生物信息学分析

Cloning and Bioinformatics Analysis of CBF1 Gene in Cerasus humilis

本试验在欧李基因组研究的基础上,利用RT-PCR方法从低温处理的‘农大4号’欧李叶片中克隆到了ChCBF1基因,该基因CDS序列长度为690 bp,编码229个氨基酸的蛋白,分子量为25.10 kD,等电点为4.94,蛋白不稳定指数为58.72。二级结构主要由α-螺旋,延伸链,β-折叠和无规则卷曲构成。启动子分析表明,ChCBF1基因的启动子元件包括低温响应元件、多个光响应相关元件以及激素响应元件等一系列诱导型顺式调控元件。通过氨基酸同源比对和系统发育树分析发现,ChCBF1基因编码的氨基酸序列与桃的相似性最高,达到95%。本研究为后续逆境与休眠诱导相关方面的基因功能验证提供了帮助。

Based on data analysis of Cerasus humilis genome,this experiment used RT-PCR method to clone the ChCBF1 gene from the leaves of Cerasus humilis'Nongda No.4'that has been treated by low temperature.The CDS sequence of ChCBF1 gene was 690 bp,and 229 amino acid proteins were encoded.The molecular weight was 25.10 kD,and the isoelectric point was 4.94,and the unstable index was 58.72.The secondary structure consists of alpha helix,extended strand,beta turn and random coil.The promoter analysis shows that the promoter components of the ChCBF1 gene include a series of inductive cis-regulating elements such as low temperature response elements,multiple photoresponse-related components,and hormone response components.Through homologous amino acid sequence alignment and evolutionary tree analysis,ChCBF1 encoded amino acid sequence is most similar to the CBF1 gene of Prunus persica,reaching 95%.This study provides help for the genetic function verification of the related aspects of follow-up adversity and dormancy induction.